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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-156 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-156, RRID:AB_2140298
- Product name
- Anti-MAPK6
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Proteomic and bioinformatic analysis of epithelial tight junction reveals an unexpected cluster of synaptic molecules.
A novel mechanism for mitogen-activated protein kinase localization.
Rapid turnover of extracellular signal-regulated kinase 3 by the ubiquitin-proteasome pathway defines a novel paradigm of mitogen-activated protein kinase regulation during cellular differentiation.
Author Index for Abstracts of the American Society of Nephrology Meeting
Kidney International 1985 Jan;27(1):353-374
Tang VW
Biology direct 2006 Dec 8;1:37
Biology direct 2006 Dec 8;1:37
A novel mechanism for mitogen-activated protein kinase localization.
Bind E, Kleyner Y, Skowronska-Krawczyk D, Bien E, Dynlacht BD, Sánchez I
Molecular biology of the cell 2004 Oct;15(10):4457-66
Molecular biology of the cell 2004 Oct;15(10):4457-66
Rapid turnover of extracellular signal-regulated kinase 3 by the ubiquitin-proteasome pathway defines a novel paradigm of mitogen-activated protein kinase regulation during cellular differentiation.
Coulombe P, Rodier G, Pelletier S, Pellerin J, Meloche S
Molecular and cellular biology 2003 Jul;23(13):4542-58
Molecular and cellular biology 2003 Jul;23(13):4542-58
Author Index for Abstracts of the American Society of Nephrology Meeting
Kidney International 1985 Jan;27(1):353-374
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 83
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
