AP32807PU-N

antibody from Acris Antibodies GmbH

Targeting: MAPK6 ERK3, HsT17250, p97MAPK, PRKM6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32807PU-N

Provider

Acris Antibodies GmbH

Product name

anti MAPK6 / ERK3

Antibody type

Polyclonal

Antigen

Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Goat

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N (0.5 µg/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N (1.5 µg) Immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with Rabbit anti-Erk3 (and co-precipitation was measured using Mouse anti-MK5/PRAK in the lower panel).