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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- R34292 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MAPK6 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids HSPVGSPLKSIQ were used as the immunogen for this MAPK6 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the MAPK6 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing mouse MAPK6-GFP tested with MAPK6 antibody (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Observed molecular weight of MAPK6+ GFP: ~130kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAPK6 antibody (1.5ug) precipitate from lysates of PRAK/MAPK6 double KO mouse embryonic fibroblasts, with (third lane) and without (fourth lane) rescued PRAK/MAPK6 expression through retroviral transduction. Routinely observed molecular weight: ~97kDa.
