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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- AP32807PU-N - Provider product page
- Provider
- OriGene
- Product name
- MAPK6 goat polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- MAPK6 goat polyclonal antibody, Aff - Purified
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- MAPK6
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293 lysate (10 ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with?MAPK6 / ERK3 Antibody ?(0.5 ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- MAPK6 / ERK3 Antibody ??(1.5 ug) Immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with Rabbit anti-Erk3 (and co-precipitation was measured using Mouse anti-MK5/PRAK in the lower panel).
- Validation comment
- IP
