TA500077

antibody from OriGene

Targeting: MAPK6 ERK3, HsT17250, p97MAPK, PRKM6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500077

Provider

OriGene

Proper citation

OriGene Cat#TA500077, RRID:AB_2140415

Product name

Anti-ERK3 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1)

Antibody type

Monoclonal

Description

Anti-ERK3 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1)

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK6

Isotype

IgG

Antibody clone number

OTI5E1

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(RC201597) (RC201597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 1 cell line by using anti-MAPK6 monoclonal antibody at 1:200 dilution.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MAPK6 mouse monoclonal antibody (TA500077) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6(RC201597)

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody (TA500077).

Validation comment

IF