Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ72939 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-ERK3/MAPK6 antibody (Internal) (STJ72939)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-ERK3/MAPK6 (Internal) is suitable for use in ELISA research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
HSPVGSPLKSIQ
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with STJ72939 (0. 5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72939 (1. 5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA