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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-143005 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ERK3 in HEK293 lysate overexpressing GFP-fused Mouse ERK3 (10 µg protein in RIPA buffer) using ERK3 Polyclonal Antibody (Product # PA5-143005) (left panel) at 0.5 µg/mL and with anti-GFP (right panel). GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ERK3 in paraffin embedded Human Heart using ERK3 Polyclonal Antibody (Product # PA5-143005) at 10 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ERK3 in lysates of MK5/ERK3 double knockout MEFs using ERK3 Polyclonal Antibody (Product # PA5-143005) (1.5 µg), with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
