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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-37592 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HCK (Tyr522) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is JurKat cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references The receptor-type protein tyrosine phosphatase CD45 promotes onset and severity of IL-1β-mediated autoinflammatory osteomyelitis.
Kralova J, Pavliuchenko N, Fabisik M, Ilievova K, Spoutil F, Prochazka J, Pokorna J, Sedlacek R, Brdicka T
The Journal of biological chemistry 2021 Oct;297(4):101131
The Journal of biological chemistry 2021 Oct;297(4):101131
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Jurkat cells using HCK (pTyr521) polyclonal antibody (Product # PA5-37592) (left) or the same antibody preincubated with a blocking peptide (right).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Jurkat cells using HCK (pTyr522) polyclonal antibody (Product # PA5-37592) (left). The lane on the right is treated with the antigen-specific peptide.
