H00003055-M01

antibody from Abnova Corporation

Targeting: HCK JTK9

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003055-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003055-M01, RRID:AB_425476

Product name

HCK monoclonal antibody (M01), clone 1D9-1A9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HCK.

Antigen sequence

MGCMKSKFLQVGGNTSSKTETSASPHCPVYVPDPT
STIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEA
IHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEG
YIPSNYVARVDSLETEEWFFKGISRKDAERQLLAP
GNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVK
HYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGND
GLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEK
KLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEA
FLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFM
AKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAF
IEQRNYIHRDLRAANILVSASLVCKIADFGLARVI
EDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVW
SFGILLMEIVTYGRIPYPGVSNPEVIRALERGYRM
PRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVL
DDFYTATESQYQQQP


Isotype

IgG

Antibody clone number

1D9-1A9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of HCK transfected lysate using anti-HCK monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HCK MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol