Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003055-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003055-D01, RRID:AB_10661986
- Product name
- HCK MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human HCK protein.
- Antigen sequence
MGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPT
STIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEA
IHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEG
YIPSNYVARVDSLETEEWFFKGISRKDAERQLLAP
GNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVK
HYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGND
GLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEK
KLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEA
FLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFM
AKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAF
IEQRNYIHRDLRAANILVSASLVCKIADFGLARVI
EDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVW
SFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRM
PRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVL
DDFYTATESQYQQQP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HCK expression in transfected 293T cell line (H00003055-T02) by HCK MaxPab polyclonal antibody.Lane 1: HCK transfected lysate(57.30 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to HCK on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HCK transfected lysate using anti-HCK MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HCK purified MaxPab mouse polyclonal antibody (B01P) (H00003055-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
