MA5-15371

antibody from Invitrogen Antibodies

Targeting: HCK JTK9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15371

Provider

Invitrogen Antibodies

Product name

HCK Monoclonal Antibody (3D12E10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15371 targets HCK in IHC and WB applications and shows reactivity with Human samples.

Antibody clone number

3D12E10

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HCK using a HCK monoclonal antibody (Product # MA5-15371) against a truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP-1 (Lane 1), U-937 (Lane 2), AML-193 (Lane 3), HL-60 (Lane 4), Jurkat (Lane 5) and A549 (Lane 6). The blot was probed with Anti- HCK Monoclonal Antibody (Product # MA5-15371, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 59 kDa band corresponding to HCK was detected in THP-1, U-937, AML-193, HL-60 but not in Jurkat, A549 which are reported negative for HCK expression.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon cancer (left) and pancreas cancer (right) using HCK monoclonal antibody (Product # MA5-15371) followed with DAB staining.