Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [8]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-27103 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Drosophila, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Benzothiazole derivative bearing amide moiety induces p53-mediated apoptosis in HPV16 positive cervical cancer cells.
Mycobacterium tuberculosis PPE2 Protein Interacts with p67phox and Inhibits Reactive Oxygen Species Production.
BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.
Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NFĸB/AKT/mTOR/KEAP1 signalling in rat heart.
The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells.
Interaction of miR-125b-5p with Human antigen R mRNA: Mechanism of controlling HCV replication.
Tension-dependent regulation of mammalian Hippo signaling through LIMD1.
Exercise Training Has Contrasting Effects in Myocardial Infarction and Pressure Overload Due to Divergent Endothelial Nitric Oxide Synthase Regulation.
Serum α-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome.
Pseudoexfoliation and Alzheimer's associated CLU risk variant, rs2279590, lies within an enhancer element and regulates CLU, EPHX2 and PTK2B gene expression.
Ischemia reduces inter-alpha inhibitor proteins in the brain of the ovine fetus.
KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.
Expression of Wilms' tumor 1 (WT1) in ameloblastomas.
Curcumin Induces Apoptosis in Pre-B Acute Lymphoblastic Leukemia Cell Lines Via PARP-1 Cleavage.
Hyperbaric Oxygen Reduces Production of Reactive Oxygen Species in Neutrophils from Polytraumatized Patients Yielding in the Inhibition of p38 MAP Kinase and Downstream Pathways.
Signaling and Immunoresolving Actions of Resolvin D1 in Inflamed Human Visceral Adipose Tissue.
Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis.
Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer.
Localization of Hippo signalling complexes and Warts activation in vivo.
Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research.
Heat shock protein 90 positively regulates Chikungunya virus replication by stabilizing viral non-structural protein nsP2 during infection.
Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat.
Activating transcription factor 4 (ATF4) modulates post-synaptic development and dendritic spine morphology.
Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells.
A mutation in Ampd2 is associated with nephrotic syndrome and hypercholesterolemia in mice.
N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
Development and characterization of monoclonal antibody against non-structural protein-2 of Chikungunya virus and its application.
Modi A, Singh M, Gutti G, Shanker OR, Singh VK, Singh S, Singh SK, Pradhan S, Narayan G
Investigational new drugs 2020 Aug;38(4):934-945
Investigational new drugs 2020 Aug;38(4):934-945
Mycobacterium tuberculosis PPE2 Protein Interacts with p67phox and Inhibits Reactive Oxygen Species Production.
Srivastava S, Battu MB, Khan MZ, Nandicoori VK, Mukhopadhyay S
Journal of immunology (Baltimore, Md. : 1950) 2019 Sep 1;203(5):1218-1229
Journal of immunology (Baltimore, Md. : 1950) 2019 Sep 1;203(5):1218-1229
BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.
Eiraku N, Chiba N, Nakamura T, Amir MS, Seong CH, Ohnishi T, Kusuyama J, Noguchi K, Matsuguchi T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):12124-12134
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):12124-12134
Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NFĸB/AKT/mTOR/KEAP1 signalling in rat heart.
Mishra P, Paital B, Jena S, Swain SS, Kumar S, Yadav MK, Chainy GBN, Samanta L
Scientific reports 2019 May 15;9(1):7408
Scientific reports 2019 May 15;9(1):7408
The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells.
Winzi M, Casas Vila N, Paszkowski-Rogacz M, Ding L, Noack S, Theis M, Butter F, Buchholz F
PloS one 2018;13(1):e0191682
PloS one 2018;13(1):e0191682
Interaction of miR-125b-5p with Human antigen R mRNA: Mechanism of controlling HCV replication.
Shwetha S, Sharma G, Raheja H, Goel A, Aggarwal R, Das S
Virus research 2018 Oct 15;258:1-8
Virus research 2018 Oct 15;258:1-8
Tension-dependent regulation of mammalian Hippo signaling through LIMD1.
Ibar C, Kirichenko E, Keepers B, Enners E, Fleisch K, Irvine KD
Journal of cell science 2018 Mar 2;131(5)
Journal of cell science 2018 Mar 2;131(5)
Exercise Training Has Contrasting Effects in Myocardial Infarction and Pressure Overload Due to Divergent Endothelial Nitric Oxide Synthase Regulation.
van Deel ED, Octavia Y, de Waard MC, de Boer M, Duncker DJ
International journal of molecular sciences 2018 Jul 6;19(7)
International journal of molecular sciences 2018 Jul 6;19(7)
Serum α-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome.
Sarabhai T, Peter C, Bär AK, Windolf J, Relja B, Wesselborg S, Wahlers T, Paunel-Görgülü A
PloS one 2017;12(5):e0177450
PloS one 2017;12(5):e0177450
Pseudoexfoliation and Alzheimer's associated CLU risk variant, rs2279590, lies within an enhancer element and regulates CLU, EPHX2 and PTK2B gene expression.
Padhy B, Hayat B, Nanda GG, Mohanty PP, Alone DP
Human molecular genetics 2017 Nov 15;26(22):4519-4529
Human molecular genetics 2017 Nov 15;26(22):4519-4529
Ischemia reduces inter-alpha inhibitor proteins in the brain of the ovine fetus.
Spasova MS, Chen X, Sadowska GB, Horton ER, Lim YP, Stonestreet BS
Developmental neurobiology 2017 Jun;77(6):726-737
Developmental neurobiology 2017 Jun;77(6):726-737
KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.
Yeyati PL, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams IR, Playfoot C, Gilbert N, van Heyningen V, Wills J, von Kriegsheim A, Finch A, Sakai J, Schofield CJ, Jackson IJ, Mill P
The Journal of cell biology 2017 Apr 3;216(4):999-1013
The Journal of cell biology 2017 Apr 3;216(4):999-1013
Expression of Wilms' tumor 1 (WT1) in ameloblastomas.
Bologna-Molina R, Takeda Y, Kuga T, Chosa N, Kitagawa M, Takata T, Ishisaki A, Mikami T
Journal of oral science 2016;58(3):407-13
Journal of oral science 2016;58(3):407-13
Curcumin Induces Apoptosis in Pre-B Acute Lymphoblastic Leukemia Cell Lines Via PARP-1 Cleavage.
Mishra D, Singh S, Narayan G
Asian Pacific journal of cancer prevention : APJCP 2016;17(8):3865-9
Asian Pacific journal of cancer prevention : APJCP 2016;17(8):3865-9
Hyperbaric Oxygen Reduces Production of Reactive Oxygen Species in Neutrophils from Polytraumatized Patients Yielding in the Inhibition of p38 MAP Kinase and Downstream Pathways.
Grimberg-Peters D, Büren C, Windolf J, Wahlers T, Paunel-Görgülü A
PloS one 2016;11(8):e0161343
PloS one 2016;11(8):e0161343
Signaling and Immunoresolving Actions of Resolvin D1 in Inflamed Human Visceral Adipose Tissue.
Titos E, Rius B, López-Vicario C, Alcaraz-Quiles J, García-Alonso V, Lopategi A, Dalli J, Lozano JJ, Arroyo V, Delgado S, Serhan CN, Clària J
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3360-3370
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3360-3370
Prior regular exercise improves clinical outcome and reduces demyelination and axonal injury in experimental autoimmune encephalomyelitis.
Bernardes D, Brambilla R, Bracchi-Ricard V, Karmally S, Dellarole A, Carvalho-Tavares J, Bethea JR
Journal of neurochemistry 2016 Jan;136 Suppl 1:63-73
Journal of neurochemistry 2016 Jan;136 Suppl 1:63-73
Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer.
Srinivas C, Ramaiah MJ, Lavanya A, Yerramsetty S, Kavi Kishor PB, Basha SA, Kamal A, Bhadra U, Bhadra MP
PloS one 2015;10(11):e0142006
PloS one 2015;10(11):e0142006
Localization of Hippo signalling complexes and Warts activation in vivo.
Sun S, Reddy BV, Irvine KD
Nature communications 2015 Sep 30;6:8402
Nature communications 2015 Sep 30;6:8402
Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research.
Kumar S, Mamidi P, Kumar A, Basantray I, Bramha U, Dixit A, Maiti PK, Singh S, Suryawanshi AR, Chattopadhyay S, Chattopadhyay S
Archives of virology 2015 Nov;160(11):2749-61
Archives of virology 2015 Nov;160(11):2749-61
Heat shock protein 90 positively regulates Chikungunya virus replication by stabilizing viral non-structural protein nsP2 during infection.
Das I, Basantray I, Mamidi P, Nayak TK, B M P, Chattopadhyay S, Chattopadhyay S
PloS one 2014;9(6):e100531
PloS one 2014;9(6):e100531
Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat.
Padiya R, Chowdhury D, Borkar R, Srinivas R, Pal Bhadra M, Banerjee SK
PloS one 2014;9(5):e94228
PloS one 2014;9(5):e94228
Activating transcription factor 4 (ATF4) modulates post-synaptic development and dendritic spine morphology.
Liu J, Pasini S, Shelanski ML, Greene LA
Frontiers in cellular neuroscience 2014;8:177
Frontiers in cellular neuroscience 2014;8:177
Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells.
Pradhan N, Pratheek BM, Garai A, Kumar A, Meena VS, Ghosh S, Singh S, Kumari S, Chandrashekar TK, Goswami C, Chattopadhyay S, Kar S, Maiti PK
Cell biology international 2014 Oct;38(10):1118-31
Cell biology international 2014 Oct;38(10):1118-31
A mutation in Ampd2 is associated with nephrotic syndrome and hypercholesterolemia in mice.
Helmering J, Juan T, Li CM, Chhoa M, Baron W, Gyuris T, Richards WG, Turk JR, Lawrence J, Cosgrove PA, Busby J, Kim KW, Kaufman SA, Cummings C, Carlson G, Véniant MM, Lloyd DJ
Lipids in health and disease 2014 Oct 31;13:167
Lipids in health and disease 2014 Oct 31;13:167
N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
Mellergaard M, Skovbakke SL, Schneider CL, Lauridsen F, Andresen L, Jensen H, Skov S
The Journal of biological chemistry 2014 Jul 18;289(29):20078-91
The Journal of biological chemistry 2014 Jul 18;289(29):20078-91
Development and characterization of monoclonal antibody against non-structural protein-2 of Chikungunya virus and its application.
Chattopadhyay S, Kumar A, Mamidi P, Nayak TK, Das I, Chhatai J, Basantray I, Bramha U, Maiti PK, Singh S, Suryawanshi AR, Chattopadhyay S
Journal of virological methods 2014 Apr;199:86-94
Journal of virological methods 2014 Apr;199:86-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein in lysates of (A) HEK 293 cells and (B) Drosophila larvae using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein (theoretical molecular weight 36 kDa) in lysates of Mouse cell lines (A) NIH 3T3 (B) RAW 264.7 using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein (theoretical molecular weight: 36 kDa) in HeLa cells lysate using GAPDH antibody (clone 13H12) in (A) the absence and (B) the presence of immunizing peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - analysis of GAPDH in HeLa and HEK 293 cells (25ug/lane) using anti-GAPDH antibody. Image from verified customer review.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH served as a loading control in a western blot of HuR knockdown and overexpression. Citation: Winzi M, Casas Vila N, Paszkowski-Rogacz M, Ding L, Noack S, Theis M, et al. (2018) The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells. PLoS ONE 13(1): e0191682. https://doi.org/10.1371/journal.pone.0191682
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - Characterization of lncR492. (B) Northern blot of lncR494 using increasing amounts of loaded total. Black arrow signifies lncR492-specific signal at approximately 1400 bp. A probe targeting Gapdh mRNA served as the loading control. (D) RT-PCR analysis of lncR492 and Gapdh expression. RNA extract was treated with a 5'-phosphate-dependent exonuclease, resulting in a degradation of f.ex. ribosomal RNA. Citation: Winzi M, Casas Vila N, Paszkowski-Rogacz M, Ding L, Noack S, Theis M, et al. (2018) The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells. PLoS ONE 13(1): e0191682. https://doi.org/10.1371/journal.pone.0191682
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH/G3PDH Antibody (13H12) [NBP2-27103] - Simple Western lane view shows a specific band for GAPDH in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - Serum from trauma patients induces intrinsic apoptosis resistance by up-regulating AAT expression in neutrophils. AAT protein expression was analyzed in neutrophils after 18 h of culture by western blot analysis. Relative expression was quantified vs. GAPDH expression. One representative blot of five independent experiments is depicted. **p
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human colon tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human rectal carcinoma tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human breast tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human stomach tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.