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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN1580424 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- conc. tissue culture supernatant
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- 1D4
- Vial size
- 500 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- blots of crude extract of peripheral nerve of various knock out mice strains blotted with ABIN1580424 for use as a western blotting control. Note the single sharp clean band at 38 kDa corresponding to GAPDH.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Human neuroblastoma SH-SY5Y cells stained with ABIN1580424 (green), chicken antibody to neurofilament NF-H CPCA-NF-H (red) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH. Some of the cells are also rich in NF-H.
