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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- AP7873a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7873a, RRID:AB_2107461
- Product name
- GAPDH Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer.
MicroRNAs are involved in erythroid differentiation control.
Wan F, Qin X, Zhang G, Lu X, Zhu Y, Zhang H, Dai B, Shi G, Ye D
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Jan 8;
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Jan 8;
MicroRNAs are involved in erythroid differentiation control.
Yang GH, Wang F, Yu J, Wang XS, Yuan JY, Zhang JW
Journal of cellular biochemistry 2009 Jun 1;107(3):548-56
Journal of cellular biochemistry 2009 Jun 1;107(3):548-56
No comments: Submit comment
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (N-term)(Cat. #AP7873a).AP7873a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of GAPDH Antibody (N-term) (Cat.#AP7873a) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAPDH Antibody (N-term)(Cat#AP7873a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- GAPDH Antibody (N-term) (Cat. # AP7873a) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
