Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA040067 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA040067, RRID:AB_10965903
- Product name
- Anti-GAPDH
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GAPDH, Gene description: glyceraldehyde-3-phosphate dehydrogenase, Alternative Gene Names: GAPD, Validated applications: ICC, IHC, WB, Uniprot ID: P04406, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Claudin-4 Stabilizes the Genome via Nuclear and Cell-Cycle Remodeling to Support Ovarian Cancer Cell Survival
Differential requirements for Smarca5 expression during hematopoietic stem cell commitment
Nrf2 Plays a Key Role in Erythropoiesis during Aging
Depletion of the RNA-binding protein PURA triggers changes in posttranscriptional gene regulation and loss of P-bodies
C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization
Mass spectrometry-based proteomic strategy for ecchymotic skin examination in forensic pathology
Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors
Inhibition of SND1 overcomes chemoresistance in bladder cancer cells by promoting ferroptosis
Chromatin Remodeler Smarca5 Is Required for Cancer-Related Processes of Primary Cell Fitness and Immortalization
The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation
Effect of Lycopene on Oral Squamous Cell Carcinoma Cell Growth by Inhibiting IGF1 Pathway.
NAD-dependent methylenetetrahydrofolate dehydrogenase inhibits oral squamous cell carcinoma cell proliferation and promotes apoptosis.
Glycolytic biomarkers predict transformation in patients with follicular lymphoma.
Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against Ischemia/Reperfusion Injury in Mice
Hepatic miR‑215 target Rictor and modulation of hepatic insulin signalling in rats
Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genes
Moderate activation of IKK2-NF-kB in unstressed adult mouse liver induces cytoprotective genes and lipogenesis without apparent signs of inflammation or fibrosis
Villagomez F, Lang J, Nunez-Avellaneda D, Behbakht K, Dimmick H, Webb P, Nephew K, Neville M, Woodruff E, Bitler B
Cancer Research Communications 2025;5(1):39-53
Cancer Research Communications 2025;5(1):39-53
Differential requirements for Smarca5 expression during hematopoietic stem cell commitment
Turkova T, Kokavec J, Zikmund T, Dibus N, Pimkova K, Nemec D, Holeckova M, Ruskova L, Sedlacek R, Cermak L, Stopka T
Communications Biology 2024;7(1)
Communications Biology 2024;7(1)
Nrf2 Plays a Key Role in Erythropoiesis during Aging
Mbiandjeu S, Siciliano A, Mattè A, Federti E, Perduca M, Melisi D, Andolfo I, Amoresano A, Iolascon A, Valenti M, Turrini F, Bovi M, Pisani A, Recchiuti A, Mattoscio D, Riccardi V, Dalle Carbonare L, Brugnara C, Mohandas N, De Franceschi L
Antioxidants 2024;13(4):454
Antioxidants 2024;13(4):454
Villagomez F, Lang J, Nunez-Avellaneda D, Behbakht K, Dimmick H, Webb P, Nephew K, Neville M, Woodruff E, Bitler B
2024
2024
Depletion of the RNA-binding protein PURA triggers changes in posttranscriptional gene regulation and loss of P-bodies
Molitor L, Klostermann M, Bacher S, Merl-Pham J, Spranger N, Burczyk S, Ketteler C, Rusha E, Tews D, Pertek A, Proske M, Busch A, Reschke S, Feederle R, Hauck S, Blum H, Drukker M, Fischer-Posovszky P, König J, Zarnack K, Niessing D
Nucleic Acids Research 2023;51(3):1297-1316
Nucleic Acids Research 2023;51(3):1297-1316
C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization
Desmurs M, Foti M, Raemy E, Vaz F, Martinou J, Bairoch A, Lane L
Molecular and Cellular Biology 2023;35(7):1139-1156
Molecular and Cellular Biology 2023;35(7):1139-1156
Mass spectrometry-based proteomic strategy for ecchymotic skin examination in forensic pathology
Toma L, Vignali G, Maffioli E, Tambuzzi S, Giaccari R, Mattarozzi M, Nonnis S, Milioli M, Franceschetti L, Paredi G, Negri A, Riccardi B, Cattaneo C, Careri M, Tedeschi G, Bruno S
Scientific Reports 2023;13(1)
Scientific Reports 2023;13(1)
Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors
Lappin K, Barros E, Jhujh S, Irwin G, McMillan H, Liberante F, Latimer C, La Bonte M, Mills K, Harkin D, Stewart G, Savage K
Cancer Research 2022;82(5):819-830
Cancer Research 2022;82(5):819-830
Inhibition of SND1 overcomes chemoresistance in bladder cancer cells by promoting ferroptosis
Zhao Y, Ren P, Yang Z, Wang L, Hu C
Oncology Reports 2022;49(1)
Oncology Reports 2022;49(1)
Chromatin Remodeler Smarca5 Is Required for Cancer-Related Processes of Primary Cell Fitness and Immortalization
Thakur S, Cahais V, Turkova T, Zikmund T, Renard C, Stopka T, Korenjak M, Zavadil J
Cells 2022;11(5):808
Cells 2022;11(5):808
The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation
Yang R, Liang X, Wang H, Guo M, Shen H, Shi Y, Liu Q, Sun Y, Yang L, Zhan M
EBioMedicine 2021;63
EBioMedicine 2021;63
Effect of Lycopene on Oral Squamous Cell Carcinoma Cell Growth by Inhibiting IGF1 Pathway.
Tao A, Wang X, Li C
Cancer management and research 2021;13:723-732
Cancer management and research 2021;13:723-732
NAD-dependent methylenetetrahydrofolate dehydrogenase inhibits oral squamous cell carcinoma cell proliferation and promotes apoptosis.
Zhao L, Cheng Z, Lu Z, Jin J
Translational cancer research 2021 Mar;10(3):1457-1469
Translational cancer research 2021 Mar;10(3):1457-1469
Glycolytic biomarkers predict transformation in patients with follicular lymphoma.
Monrad I, Madsen C, Lauridsen KL, Honoré B, Plesner TL, Hamilton-Dutoit S, d'Amore F, Ludvigsen M
PloS one 2020;15(5):e0233449
PloS one 2020;15(5):e0233449
Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against Ischemia/Reperfusion Injury in Mice
Shao J, Miao C, Geng Z, Gu M, Wu Y, Li Q
BioMed Research International 2019;2019
BioMed Research International 2019;2019
Hepatic miR‑215 target Rictor and modulation of hepatic insulin signalling in rats
Li W, Xia J, Lian Y
Molecular Medicine Reports 2019
Molecular Medicine Reports 2019
Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genes
Lu H, Lei X, Zhang Q
Xenobiotica 2018;49(6):740-752
Xenobiotica 2018;49(6):740-752
Moderate activation of IKK2-NF-kB in unstressed adult mouse liver induces cytoprotective genes and lipogenesis without apparent signs of inflammation or fibrosis
Lu H, Lei X, Zhang Q
BMC Gastroenterology 2015;15(1)
BMC Gastroenterology 2015;15(1)
No comments: Submit comment
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
