Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF5718 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal GAPDH Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GAPDH-2 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey.
PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo.
Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway.
Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism.
Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium.
Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice.
Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis.
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy.
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.
Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice.
Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function.
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Overexpression of carbonic anhydrase and HIF-1α in Wilms tumours.
Tan J, Wang X, Cai S, He F, Zhang D, Li D, Zhu X, Zhou L, Fan N, Liu X
Molecular therapy. Methods & clinical development 2020 Jun 12;17:143-155
Molecular therapy. Methods & clinical development 2020 Jun 12;17:143-155
PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo.
Liu L, Cao J, Zhao J, Li X, Suo Z, Li H
OncoTargets and therapy 2019;12:9899-9913
OncoTargets and therapy 2019;12:9899-9913
Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway.
Der Vartanian A, Chabanais J, Carrion C, Maftah A, Germot A
International journal of molecular sciences 2019 Sep 6;20(18)
International journal of molecular sciences 2019 Sep 6;20(18)
Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism.
Liu T, Barrett NA, Kanaoka Y, Buchheit K, Laidlaw TM, Garofalo D, Lai J, Katz HR, Feng C, Boyce JA
Mucosal immunology 2019 May;12(3):679-690
Mucosal immunology 2019 May;12(3):679-690
Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium.
Ilyas B, Mulder DT, Little DJ, Elhenawy W, Banda MM, Pérez-Morales D, Tsai CN, Chau NYE, Bustamante VH, Coombes BK
Cell reports 2018 Oct 23;25(4):825-832.e5
Cell reports 2018 Oct 23;25(4):825-832.e5
Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice.
Périè L, Parenté A, Baraige F, Magnol L, Blanquet V
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(5):1896-1911
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(5):1896-1911
Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis.
Shim MK, Yoon HY, Lee S, Jo MK, Park J, Kim JH, Jeong SY, Kwon IC, Kim K
Scientific reports 2017 Nov 30;7(1):16635
Scientific reports 2017 Nov 30;7(1):16635
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, Wang J, Zhang H, Berge V, Goscinski MA, Kvalheim G, Nesland JM, Suo Z
Scientific reports 2017 Jul 11;7(1):5081
Scientific reports 2017 Jul 11;7(1):5081
Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy.
Ganesan R, Hos NJ, Gutierrez S, Fischer J, Stepek JM, Daglidu E, Krönke M, Robinson N
PLoS pathogens 2017 Feb;13(2):e1006227
PLoS pathogens 2017 Feb;13(2):e1006227
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.
Yang CT, Ma R, Axton RA, Jackson M, Taylor AH, Fidanza A, Marenah L, Frayne J, Mountford JC, Forrester LM
Stem cells (Dayton, Ohio) 2017 Apr;35(4):886-897
Stem cells (Dayton, Ohio) 2017 Apr;35(4):886-897
Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
Investigative ophthalmology & visual science 2014 May 27;55(7):4276-87
Investigative ophthalmology & visual science 2014 May 27;55(7):4276-87
Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice.
Chen F, Marquez H, Kim YK, Qian J, Shao F, Fine A, Cruikshank WW, Quadro L, Cardoso WV
The Journal of clinical investigation 2014 Feb;124(2):801-11
The Journal of clinical investigation 2014 Feb;124(2):801-11
Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function.
Zhi H, Luptak I, Alreja G, Shi J, Guan J, Metes-Kosik N, Joseph J
PloS one 2013;8(12):e81612
PloS one 2013;8(12):e81612
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
BMC cancer 2012 May 29;12:201
BMC cancer 2012 May 29;12:201
Overexpression of carbonic anhydrase and HIF-1α in Wilms tumours.
Dungwa JV, Hunt LP, Ramani P
BMC cancer 2011 Sep 12;11:390
BMC cancer 2011 Sep 12;11:390
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human GAPDH by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GAPDH in Human Prostate Cancer Tissue. GAPDH was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.