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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- ACR001PS - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti GAPDH (Loading Control)
- Antibody type
- Monoclonal
- Antigen
- Rabbit GAPDH.
- Reactivity
- Human, Mouse, Rat, Canine, Feline, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6C5
- Vial size
- 0.1 mg
- Concentration
- lot specific
Submitted references Aryl hydrocarbon receptor is critical for homeostasis of invariant gammadelta T cells in the murine epidermis.
Langerhans cell maturation and contact hypersensitivity are impaired in aryl hydrocarbon receptor-null mice.
Signal transduction and transcriptional control of cardiac connexin43 up-regulation after alpha 1-adrenoceptor stimulation.
Kadow S, Jux B, Zahner SP, Wingerath B, Chmill S, Clausen BE, Hengstler J, Esser C
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3104-10
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3104-10
Langerhans cell maturation and contact hypersensitivity are impaired in aryl hydrocarbon receptor-null mice.
Jux B, Kadow S, Esser C
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):6709-17
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):6709-17
Signal transduction and transcriptional control of cardiac connexin43 up-regulation after alpha 1-adrenoceptor stimulation.
Salameh A, Krautblatter S, Baessler S, Karl S, Rojas Gomez D, Dhein S, Pfeiffer D
The Journal of pharmacology and experimental therapeutics 2008 Jul;326(1):315-22
The Journal of pharmacology and experimental therapeutics 2008 Jul;326(1):315-22
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blotting for GAPDH on Rat Brain lysates using anti-GAPDH monoclonal antibody Cat.-No ACR001P at 1 µg/ml (Top) and 0.1 µg/ml (Bottom).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blotting for GAPDH on HUVEC cell lysate using anti-GAPDH monoclonal antibody Cat.-No ACR001P at 2 µg/ml (A and B) and 0.5 µg/ml (C and D).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Staining of Rh30 rhabdomyosarcoma (upper panels) and SJSA-1 Ewingâs sarcoma cells (lower panels) for GAPDH with ACR001P anti-GAPDH monoclonal antibody (left panels). FITC conjugated secondary antibody. The middle panels show DAPI staining of the cell nuclei. The right panels show merged images.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5: Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis. Track 1: Human GAPDH (1 μg). Track 2: GAPDH immunoprecipitated from rat heart tissue extract. Track 3: Only MAb 6C5 (A) or 4G5 (B) preincubated with Protein A Sepharose. Track 4: Only Protein A Sepharose.
