Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-37828 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This GAPDH antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples. This product has been successfully used in Western blot, ELISA, and immunofluorescence.
- Reactivity
- Human, Mouse, Rat, Bacteria, Chicken/Avian, Hamster, Porcine, Rabbit, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Myocardin and myocardin-related transcription factor-A synergistically mediate actin cytoskeletal-dependent inhibition of liver fibrogenesis.
Shi Z, Ren M, Rockey DC
American journal of physiology. Gastrointestinal and liver physiology 2020 Mar 1;318(3):G504-G517
American journal of physiology. Gastrointestinal and liver physiology 2020 Mar 1;318(3):G504-G517
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).