Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG63542 - Provider product page
- Provider
- Arigo
- Product name
- anti-GAPDH antibody
- Antibody type
- Polyclonal
- Antigen
- C-HQVVSSDFNSDT
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35 µg protein in RIPA buffer) Level of knock-down relative to Actin expression level was determined by RT-PCR. stained with ARG63542 anti-GAPDH antibody at 0.1 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HEK293 cell lysate (35 µg protein in RIPA buffer). stained with ARG63542 anti-GAPDH antibody at 0.03 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson stained with ARG63542 anti-GAPDH antibody (0.1ug/ml). Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).