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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [4]
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- Product number
- NB300-327 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-327, RRID:AB_10001915
- Product name
- Rabbit Polyclonal GAPDH Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Bacteria, Bovine, Chicken/Avian, Porcine, Yeast
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Dual histone methyl reader ZCWPW1 facilitates repair of meiotic double strand breaks in male mice.
Histological hallmarks and role of Slug/PIP axis in pulmonary hypertension secondary to pulmonary fibrosis.
Keeping Candida commensal: how lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model.
Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells.
Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.
Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia.
Ovatodiolide suppresses allergic airway inflammation and hyperresponsiveness in a murine model of asthma.
Endothelin-1 down-regulates matrix metalloproteinase 14 and 15 expression in human first trimester trophoblasts via endothelin receptor type B.
A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.
Effect Of combining FK506 and neurotrophins on neurite branching and elongation.
Botulinum toxin type A reduces inflammatory hypernociception induced by arthritis in the temporomadibular joint of rats.
Characterization of ATP7A missense mutants suggests a correlation between intracellular trafficking and severity of Menkes disease.
Exogenous Modulation of Retinoic Acid Signaling Affects Adult RGC Survival in the Frog Visual System after Optic Nerve Injury.
Identification of TMEM230 mutations in familial Parkinson's disease.
Rescue of Pressure Overload-Induced Heart Failure by Estrogen Therapy.
In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma.
Expanding the clinical and molecular characteristics of PIGT-CDG, a disorder of glycosylphosphatidylinositol anchors.
Stabilization of HIF-2α through redox regulation of mTORC2 activation and initiation of mRNA translation.
Three prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth.
Notch signaling regulates tumor-induced angiogenesis in SPARC-overexpressed neuroblastoma.
Comparison of anti-angiogenic properties of pristine carbon nanoparticles.
Curcumin protects against thioacetamide-induced hepatic fibrosis by attenuating the inflammatory response and inducing apoptosis of damaged hepatocytes.
Sorafenib and Mek inhibition is synergistic in medullary thyroid carcinoma in vitro.
Mthfs is an Essential Gene in Mice and a Component of the Purinosome.
Akt1 deficiency delays tumor progression, vascular invasion, and distant metastasis in a murine model of thyroid cancer.
NAD(P)H oxidase mediates TGF-beta1-induced activation of kidney myofibroblasts.
Group I p21-activated kinases regulate thyroid cancer cell migration and are overexpressed and activated in thyroid cancer invasion.
A proteomic approach to the identification of tegumental proteins of male and female Schistosoma bovis worms.
Lyn kinase promotes erythroblast expansion and late-stage development.
Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion.
Mahgoub M, Paiano J, Bruno M, Wu W, Pathuri S, Zhang X, Ralls S, Cheng X, Nussenzweig A, Macfarlan TS
eLife 2020 Apr 30;9
eLife 2020 Apr 30;9
Histological hallmarks and role of Slug/PIP axis in pulmonary hypertension secondary to pulmonary fibrosis.
Ruffenach G, Umar S, Vaillancourt M, Hong J, Cao N, Sarji S, Moazeni S, Cunningham CM, Ardehali A, Reddy ST, Saggar R, Fishbein G, Eghbali M
EMBO molecular medicine 2019 Sep;11(9):e10061
EMBO molecular medicine 2019 Sep;11(9):e10061
Keeping Candida commensal: how lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model.
Graf K, Last A, Gratz R, Allert S, Linde S, Westermann M, Gröger M, Mosig AS, Gresnigt MS, Hube B
Disease models & mechanisms 2019 Sep 12;12(9)
Disease models & mechanisms 2019 Sep 12;12(9)
Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells.
Jaworski S, Strojny B, Sawosz E, Wierzbicki M, Grodzik M, Kutwin M, Daniluk K, Chwalibog A
International journal of molecular sciences 2019 Feb 2;20(3)
International journal of molecular sciences 2019 Feb 2;20(3)
Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.
Warkocki Z, Krawczyk PS, Adamska D, Bijata K, Garcia-Perez JL, Dziembowski A
Cell 2018 Sep 6;174(6):1537-1548.e29
Cell 2018 Sep 6;174(6):1537-1548.e29
Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia.
Konopaske GT, Balu DT, Presti KT, Chan G, Benes FM, Coyle JT
Schizophrenia research 2018 Nov;201:270-277
Schizophrenia research 2018 Nov;201:270-277
Ovatodiolide suppresses allergic airway inflammation and hyperresponsiveness in a murine model of asthma.
Wang CN, Lee YL, Lin YP, Chung WH, Tzeng YM, Lee CC
European journal of pharmacology 2017 Oct 5;812:9-17
European journal of pharmacology 2017 Oct 5;812:9-17
Endothelin-1 down-regulates matrix metalloproteinase 14 and 15 expression in human first trimester trophoblasts via endothelin receptor type B.
Majali-Martinez A, Velicky P, Pollheimer J, Knöfler M, Yung HW, Burton GJ, Tabrizi-Wizsy NG, Lang U, Hiden U, Desoye G, Dieber-Rotheneder M
Human reproduction (Oxford, England) 2017 Jan;32(1):46-54
Human reproduction (Oxford, England) 2017 Jan;32(1):46-54
A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.
Kalisiak K, Kuliński TM, Tomecki R, Cysewski D, Pietras Z, Chlebowski A, Kowalska K, Dziembowski A
Nucleic acids research 2017 Feb 28;45(4):2068-2080
Nucleic acids research 2017 Feb 28;45(4):2068-2080
Effect Of combining FK506 and neurotrophins on neurite branching and elongation.
Labroo P, Shea J, Sant H, Gale B, Agarwal J
Muscle & nerve 2017 Apr;55(4):570-581
Muscle & nerve 2017 Apr;55(4):570-581
Botulinum toxin type A reduces inflammatory hypernociception induced by arthritis in the temporomadibular joint of rats.
Lora VR, Clemente-Napimoga JT, Abdalla HB, Macedo CG, Canales GT, Barbosa CM
Toxicon : official journal of the International Society on Toxinology 2017 Apr;129:52-57
Toxicon : official journal of the International Society on Toxinology 2017 Apr;129:52-57
Characterization of ATP7A missense mutants suggests a correlation between intracellular trafficking and severity of Menkes disease.
Skjørringe T, Amstrup Pedersen P, Salling Thorborg S, Nissen P, Gourdon P, Birk Møller L
Scientific reports 2017 Apr 7;7(1):757
Scientific reports 2017 Apr 7;7(1):757
Exogenous Modulation of Retinoic Acid Signaling Affects Adult RGC Survival in the Frog Visual System after Optic Nerve Injury.
Duprey-Díaz MV, Blagburn JM, Blanco RE
PloS one 2016;11(9):e0162626
PloS one 2016;11(9):e0162626
Identification of TMEM230 mutations in familial Parkinson's disease.
Deng HX, Shi Y, Yang Y, Ahmeti KB, Miller N, Huang C, Cheng L, Zhai H, Deng S, Nuytemans K, Corbett NJ, Kim MJ, Deng H, Tang B, Yang Z, Xu Y, Chan P, Huang B, Gao XP, Song Z, Liu Z, Fecto F, Siddique N, Foroud T, Jankovic J, Ghetti B, Nicholson DA, Krainc D, Melen O, Vance JM, Pericak-Vance MA, Ma YC, Rajput AH, Siddique T
Nature genetics 2016 Jul;48(7):733-9
Nature genetics 2016 Jul;48(7):733-9
Rescue of Pressure Overload-Induced Heart Failure by Estrogen Therapy.
Iorga A, Li J, Sharma S, Umar S, Bopassa JC, Nadadur RD, Centala A, Ren S, Saito T, Toro L, Wang Y, Stefani E, Eghbali M
Journal of the American Heart Association 2016 Jan 22;5(1)
Journal of the American Heart Association 2016 Jan 22;5(1)
In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma.
Jaworski S, Sawosz E, Kutwin M, Wierzbicki M, Hinzmann M, Grodzik M, Winnicka A, Lipińska L, Włodyga K, Chwalibog A
International journal of nanomedicine 2015;10:1585-96
International journal of nanomedicine 2015;10:1585-96
Expanding the clinical and molecular characteristics of PIGT-CDG, a disorder of glycosylphosphatidylinositol anchors.
Lam C, Golas GA, Davids M, Huizing M, Kane MS, Krasnewich DM, Malicdan MCV, Adams DR, Markello TC, Zein WM, Gropman AL, Lodish MB, Stratakis CA, Maric I, Rosenzweig SD, Baker EH, Ferreira CR, Danylchuk NR, Kahler S, Garnica AD, Bradley Schaefer G, Boerkoel CF, Gahl WA, Wolfe LA
Molecular genetics and metabolism 2015 Jun-Jul;115(2-3):128-140
Molecular genetics and metabolism 2015 Jun-Jul;115(2-3):128-140
Stabilization of HIF-2α through redox regulation of mTORC2 activation and initiation of mRNA translation.
Nayak BK, Feliers D, Sudarshan S, Friedrichs WE, Day RT, New DD, Fitzgerald JP, Eid A, Denapoli T, Parekh DJ, Gorin Y, Block K
Oncogene 2013 Jun 27;32(26):3147-55
Oncogene 2013 Jun 27;32(26):3147-55
Three prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth.
Johnston DA, Tapia AL, Eberle KE, Palmer GE
Eukaryotic cell 2013 Jul;12(7):1039-50
Eukaryotic cell 2013 Jul;12(7):1039-50
Notch signaling regulates tumor-induced angiogenesis in SPARC-overexpressed neuroblastoma.
Gorantla B, Bhoopathi P, Chetty C, Gogineni VR, Sailaja GS, Gondi CS, Rao JS
Angiogenesis 2013 Jan;16(1):85-100
Angiogenesis 2013 Jan;16(1):85-100
Comparison of anti-angiogenic properties of pristine carbon nanoparticles.
Wierzbicki M, Sawosz E, Grodzik M, Prasek M, Jaworski S, Chwalibog A
Nanoscale research letters 2013 Apr 26;8(1):195
Nanoscale research letters 2013 Apr 26;8(1):195
Curcumin protects against thioacetamide-induced hepatic fibrosis by attenuating the inflammatory response and inducing apoptosis of damaged hepatocytes.
Wang ME, Chen YC, Chen IS, Hsieh SC, Chen SS, Chiu CH
The Journal of nutritional biochemistry 2012 Oct;23(10):1352-66
The Journal of nutritional biochemistry 2012 Oct;23(10):1352-66
Sorafenib and Mek inhibition is synergistic in medullary thyroid carcinoma in vitro.
Koh YW, Shah MH, Agarwal K, McCarty SK, Koo BS, Brendel VJ, Wang C, Porter K, Jarjoura D, Saji M, Ringel MD
Endocrine-related cancer 2012 Feb;19(1):29-38
Endocrine-related cancer 2012 Feb;19(1):29-38
Mthfs is an Essential Gene in Mice and a Component of the Purinosome.
Field MS, Anderson DD, Stover PJ
Frontiers in genetics 2011;2:36
Frontiers in genetics 2011;2:36
Akt1 deficiency delays tumor progression, vascular invasion, and distant metastasis in a murine model of thyroid cancer.
Saji M, Narahara K, McCarty SK, Vasko VV, La Perle KM, Porter K, Jarjoura D, Lu C, Cheng SY, Ringel MD
Oncogene 2011 Oct 20;30(42):4307-15
Oncogene 2011 Oct 20;30(42):4307-15
NAD(P)H oxidase mediates TGF-beta1-induced activation of kidney myofibroblasts.
Bondi CD, Manickam N, Lee DY, Block K, Gorin Y, Abboud HE, Barnes JL
Journal of the American Society of Nephrology : JASN 2010 Jan;21(1):93-102
Journal of the American Society of Nephrology : JASN 2010 Jan;21(1):93-102
Group I p21-activated kinases regulate thyroid cancer cell migration and are overexpressed and activated in thyroid cancer invasion.
McCarty SK, Saji M, Zhang X, Jarjoura D, Fusco A, Vasko VV, Ringel MD
Endocrine-related cancer 2010 Dec;17(4):989-99
Endocrine-related cancer 2010 Dec;17(4):989-99
A proteomic approach to the identification of tegumental proteins of male and female Schistosoma bovis worms.
Pérez-Sánchez R, Valero ML, Ramajo-Hernández A, Siles-Lucas M, Ramajo-Martín V, Oleaga A
Molecular and biochemical parasitology 2008 Oct;161(2):112-23
Molecular and biochemical parasitology 2008 Oct;161(2):112-23
Lyn kinase promotes erythroblast expansion and late-stage development.
Karur VG, Lowell CA, Besmer P, Agosti V, Wojchowski DM
Blood 2006 Sep 1;108(5):1524-32
Blood 2006 Sep 1;108(5):1524-32
Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion.
Fatma S, Yakubov R, Anwar K, Hussain MM
Journal of lipid research 2006 Nov;47(11):2422-32
Journal of lipid research 2006 Nov;47(11):2422-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-327] - Theoretical molecular weight: 36 kDa. Detection of GAPDH in mouse liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-327] - Western blot analysis of extracts from Jurkat and HeLa cells using NB300-327 at 1:1000. Theoretical molecular weight: 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody [NB300-327] - Simple Western lane view shows a specific band for GAPDH in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higer than predicted 36 kDa molecular weight.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-327] - Analysis of different cell cytosolic or nuclear enriched fractions, GAPDH antibody, dilution 1:20,000 (Red): [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse mAb to the nuclear RNA binding protein SF3B4, dilution 1:1,000 (Green). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.
