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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [4]
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- Product number
- NB300-285 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-285, RRID:AB_2107603
- Product name
- Mouse Monoclonal GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Orphan Gpr182 suppresses ERK-mediated intestinal proliferation during regeneration and adenoma formation.
Identification of CCL2, RARRES2 and EFNB2 as host cell factors that influence the multistep replication of respiratory syncytial virus.
Suppression of BRCA1 sensitizes cells to proteasome inhibitors.
Discovery of somatic STAT5b mutations in large granular lymphocytic leukemia.
Differential expression of the pro-natriuretic peptide convertases corin and furin in experimental heart failure and atrial fibrosis.
Reduced DICER1 elicits an interferon response in endometrial cancer cells.
Complete characterization of the microRNAome in a patient with acute myeloid leukemia.
Restoration of tissue factor pathway inhibitor-2 in a human glioblastoma cell line triggers caspase-mediated pathway and apoptosis.
Drug sensitivity prediction by CpG island methylation profile in the NCI-60 cancer cell line panel.
Kechele DO, Blue RE, Zwarycz B, Espenschied ST, Mah AT, Siegel MB, Perou CM, Ding S, Magness ST, Lund PK, Caron KM
The Journal of clinical investigation 2017 Feb 1;127(2):593-607
The Journal of clinical investigation 2017 Feb 1;127(2):593-607
Identification of CCL2, RARRES2 and EFNB2 as host cell factors that influence the multistep replication of respiratory syncytial virus.
Shirato K, Ujike M, Kawase M, Matsuyama S
Virus research 2015 Dec 2;210:213-26
Virus research 2015 Dec 2;210:213-26
Suppression of BRCA1 sensitizes cells to proteasome inhibitors.
Gu Y, Bouwman P, Greco D, Saarela J, Yadav B, Jonkers J, Kuznetsov SG
Cell death & disease 2014 Dec 18;5(12):e1580
Cell death & disease 2014 Dec 18;5(12):e1580
Discovery of somatic STAT5b mutations in large granular lymphocytic leukemia.
Rajala HL, Eldfors S, Kuusanmäki H, van Adrichem AJ, Olson T, Lagström S, Andersson EI, Jerez A, Clemente MJ, Yan Y, Zhang D, Awwad A, Ellonen P, Kallioniemi O, Wennerberg K, Porkka K, Maciejewski JP, Loughran TP Jr, Heckman C, Mustjoki S
Blood 2013 May 30;121(22):4541-50
Blood 2013 May 30;121(22):4541-50
Differential expression of the pro-natriuretic peptide convertases corin and furin in experimental heart failure and atrial fibrosis.
Ichiki T, Boerrigter G, Huntley BK, Sangaralingham SJ, McKie PM, Harty GJ, Harders GE, Burnett JC Jr
American journal of physiology. Regulatory, integrative and comparative physiology 2013 Jan 15;304(2):R102-9
American journal of physiology. Regulatory, integrative and comparative physiology 2013 Jan 15;304(2):R102-9
Reduced DICER1 elicits an interferon response in endometrial cancer cells.
Chiappinelli KB, Haynes BC, Brent MR, Goodfellow PJ
Molecular cancer research : MCR 2012 Mar;10(3):316-25
Molecular cancer research : MCR 2012 Mar;10(3):316-25
Complete characterization of the microRNAome in a patient with acute myeloid leukemia.
Ramsingh G, Koboldt DC, Trissal M, Chiappinelli KB, Wylie T, Koul S, Chang LW, Nagarajan R, Fehniger TA, Goodfellow P, Magrini V, Wilson RK, Ding L, Ley TJ, Mardis ER, Link DC
Blood 2010 Dec 9;116(24):5316-26
Blood 2010 Dec 9;116(24):5316-26
Restoration of tissue factor pathway inhibitor-2 in a human glioblastoma cell line triggers caspase-mediated pathway and apoptosis.
George J, Gondi CS, Dinh DH, Gujrati M, Rao JS
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jun 15;13(12):3507-17
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jun 15;13(12):3507-17
Drug sensitivity prediction by CpG island methylation profile in the NCI-60 cancer cell line panel.
Shen L, Kondo Y, Ahmed S, Boumber Y, Konishi K, Guo Y, Chen X, Vilaythong JN, Issa JP
Cancer research 2007 Dec 1;67(23):11335-43
Cancer research 2007 Dec 1;67(23):11335-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (NB615) [NB300-285] - Analysis of HeLa, Jurkat, and COS lysate using GAPDH (NB300-285) antibody at 1:100. Theoretical molecular weight: 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (NB615) [NB300-285] - Analysis of 40ug HeLa whole cell lysates using NB300-285 at a dilution of 1:100. Note: band observed higher than predicted 36 kDa molecular weight.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody (NB615) [NB300-285] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (NB615) [NB300-285] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1. PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY licence.
