GTX28245

antibody from GeneTex

Targeting: GAPDH GAPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX28245

Provider

GeneTex

Proper citation

GeneTex Cat#GTX28245, RRID:AB_370675

Product name

GAPDH antibody [6C5]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Canine, Feline, Porcine, Rabbit

Host

Mouse

References

Submitted references

Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.

Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, Jacob C
Nature communications 2017 Jan 31;8:14272

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Respiratory Syncytial Virus Attachment Glycoprotein Contribution to Infection Depends on the Specific Fusion Protein.

Meng J, Hotard AL, Currier MG, Lee S, Stobart CC, Moore ML
Journal of virology 2016 Jan 1;90(1):245-53

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Breast Cancer MDA-MB-231 Cells Use Secreted Heat Shock Protein-90alpha (Hsp90α) to Survive a Hostile Hypoxic Environment.

Dong H, Zou M, Bhatia A, Jayaprakash P, Hofman F, Ying Q, Chen M, Woodley DT, Li W
Scientific reports 2016 Feb 5;6:20605

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The Impact of dUTPase on Ribonucleotide Reductase-Induced Genome Instability in Cancer Cells.

Chen CW, Tsao N, Huang LY, Yen Y, Liu X, Lehman C, Wang YH, Tseng MC, Chen YJ, Ho YC, Chen CF, Chang ZF
Cell reports 2016 Aug 2;16(5):1287-1299

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Mitogen-Activated Protein Kinase Pathway: Genetic Analysis of 95 Adrenocortical Tumors.

Rubin B, Monticelli H, Redaelli M, Mucignat C, Barollo S, Bertazza L, Mian C, Betterle C, Iacobone M, Fassina A, Boscaro M, Pezzani R, Mantero F
Cancer investigation 2015;33(10):526-31

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Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.

Jayaprakash P, Dong H, Zou M, Bhatia A, O'Brien K, Chen M, Woodley DT, Li W
Journal of cell science 2015 Apr 15;128(8):1475-80

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Refining the balance of attenuation and immunogenicity of respiratory syncytial virus by targeted codon deoptimization of virulence genes.

Meng J, Lee S, Hotard AL, Moore ML
mBio 2014 Sep 23;5(5):e01704-14

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Sphingosine-1-phosphate induces pro-remodelling response in airway smooth muscle cells.

Fuerst E, Foster HR, Ward JP, Corrigan CJ, Cousins DJ, Woszczek G
Allergy 2014 Nov;69(11):1531-9

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Angiotensin II-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model.

Kiryu M, Niwano S, Niwano H, Kishihara J, Aoyama Y, Fukaya H, Masaki Y, Izumi T
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology 2012 Aug;14(8):1206-14

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Extra-nuclear activation of progesterone receptor in regulating arterial smooth muscle cell migration.

Hsu SP, Chen TH, Chou YP, Chen LC, Kuo CT, Lee TS, Lin JJ, Chang NC, Lee WS
Atherosclerosis 2011 Jul;217(1):83-9

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded. Lane 1: isolated human GAPDH, 0.5 ?g Lane 2: human heart tissue extract Lane 3: pig heart tissue extract Lane 4: goat heart tissue extract Lane 5: bovine heart tissue extract Lane 6: dog heart tissue extract Lane 7: mouse heart tissue extract Lane 8: rat heart tissue extract Lane 9: rabbit heart tissue extract

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by: GTX28245 (green colour) F-actin microfilaments-bindingdye (red) DNA-binding dye (dark blue)

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A). Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 ?C. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis. Lane 1: Human GAPDH (1 ?g) Lane 2: GAPDH immunoprecipitated from rat heart tissue extract Lane 3: Only GTX28245 preincubated with Protein A Sepharose Lane 4: Only Protein A Sepharose