Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP16445PU-N - Provider product page
- Provider
- OriGene
- Product name
- GAPDH goat polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- GAPDH goat polyclonal antibody, Aff - Purified
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- GAPDH
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
Submitted references Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation.
Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P
Experimental neurology 2008 Dec;214(2):347-61
Experimental neurology 2008 Dec;214(2):347-61
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody staining of Human Tonsil Lysate at 0.1 ug/ml (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody staining of Human Liver Lysate at 0.1 ug/ml (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody staining of Rat Liver Lysate at 0.5 ug/ml (RIPA buffer, 35 ug total protein per lane).?Primary incubation was overnight. Detected by alkaline phosphatase (NBT/BCIP). Data provided by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody staining of?PFA-perfused cryosection of Rat Liver at 20 ug/ml. Antigen retrieval with citrate buffer pH 3 by microwave for 20min, Cy3 detection. Data obtained by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
- Validation comment
- IHC