Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP2-59779 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human GAPDH antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2. Theoretical molecular weight: 36 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human kidney shows cytoplasmic and nuclear immunoreactivity in renal tubules and glomerular cells.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human fallopian tube shows cytoplasmic and nuclear positivity in the epithelial and connective tissue cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human liver shows cytoplasmic and nuclear immunoreactivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human pancreas shows nuclear and cytoplasmic immunoreactivity in both endocrine and exocrine cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human skeletal muscle shows moderate cytoplasmic and nuclear immunoreactivity in muscle fibers.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (CL3266) [NBP2-59779] - Staining of human pancreas shows nuclear and cytoplasmic immunoreactivity in both endocrine and exocrine cells.