Explore
Validate
Learn
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN152657 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human, Mouse, Rat, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D4A7
- Vial size
- 0.1 mL
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
Submitted references MicroRNA-552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway in colorectal cancer.
Riluzole synergizes with paclitaxel to inhibit cell growth and induce apoptosis in triple-negative breast cancer.
Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosis in cardiomyocytes.
2-(Phenylsulfonyl)quinoline N-hydroxyacrylamides as potent anticancer agents inhibiting histone deacetylase.
Riluzole mediates anti-tumor properties in breast cancer cells independent of metabotropic glutamate receptor-1.
Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation.
Interleukin-17 potently increases non-small cell lung cancer growth.
Cell stress increases ATP release in NLRP3 inflammasome-mediated autoinflammatory diseases, resulting in cytokine imbalance.
Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates.
Human mutations in methylenetetrahydrofolate dehydrogenase 1 impair nuclear de novo thymidylate biosynthesis.
Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes.
Deubiquitinating enzymes regulate PARK2-mediated mitophagy.
Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer.
Metabotropic glutamate receptor-1 contributes to progression in triple negative breast cancer.
Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress.
C10ORF10/DEPP, a transcriptional target of FOXO3, regulates ROS-sensitivity in human neuroblastoma.
Spreading of amyloid-β peptides via neuritic cell-to-cell transfer is dependent on insufficient cellular clearance.
Cortical synaptic NMDA receptor deficits in α7 nicotinic acetylcholine receptor gene deletion models: implications for neuropsychiatric diseases.
MicroRNA-340 as a modulator of RAS-RAF-MAPK signaling in melanoma.
A unifying mechanism for cancer cell death through ion channel activation by HAMLET.
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice.
Difference in protein expression profile and chemotherapy drugs response of different progression stages of LNCaP sublines and other human prostate cancer cells.
Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress.
Evidence of epidermal growth factor receptor expression in uveal melanoma: inhibition of epidermal growth factor-mediated signalling by Gefitinib and Cetuximab triggered antibody-dependent cellular cytotoxicity.
Shear stress modulates VCAM-1 expression in response to TNF-α and dietary lipids via interferon regulatory factor-1 in cultured endothelium.
Modulation of airway epithelial cell functions by Pidotimod: NF-kB cytoplasmatic expression and its nuclear translocation are associated with an increased TLR-2 expression.
Spinal serum-inducible and glucocorticoid-inducible kinase 1 mediates neuropathic pain via kalirin and downstream PSD-95-dependent NR2B phosphorylation in rats.
Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell.
Celecoxib induces proliferation and Amphiregulin production in colon subepithelial myofibroblasts, activating erk1-2 signaling in synergy with EGFR.
Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin β1.
Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers.
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Shear stress modulates RAGE-mediated inflammation in a model of diabetes-induced metabolic stress.
Regulation of neuroendocrine differentiation by AKT/hnRNPK/AR/β-catenin signaling in prostate cancer cells.
Upregulation of cellular Bcl-2 by the KSHV encoded RTA promotes virion production.
Mthfs is an Essential Gene in Mice and a Component of the Purinosome.
Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF.
Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells.
Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase.
Cao J, Yan XR, Liu T, Han XB, Yu JJ, Liu SH, Wang LB
Oncology letters 2017 Sep;14(3):3795-3802
Oncology letters 2017 Sep;14(3):3795-3802
Riluzole synergizes with paclitaxel to inhibit cell growth and induce apoptosis in triple-negative breast cancer.
Speyer CL, Bukhsh MA, Jafry WS, Sexton RE, Bandyopadhyay S, Gorski DH
Breast cancer research and treatment 2017 Nov;166(2):407-419
Breast cancer research and treatment 2017 Nov;166(2):407-419
Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosis in cardiomyocytes.
Zhao L, Zhang B
Scientific reports 2017 Mar 16;7:44735
Scientific reports 2017 Mar 16;7:44735
2-(Phenylsulfonyl)quinoline N-hydroxyacrylamides as potent anticancer agents inhibiting histone deacetylase.
Lee HY, Chang CY, Su CJ, Huang HL, Mehndiratta S, Chao YH, Hsu CM, Kumar S, Sung TY, Huang YZ, Li YH, Yang CR, Liou JP
European journal of medicinal chemistry 2016 Oct 21;122:92-101
European journal of medicinal chemistry 2016 Oct 21;122:92-101
Riluzole mediates anti-tumor properties in breast cancer cells independent of metabotropic glutamate receptor-1.
Speyer CL, Nassar MA, Hachem AH, Bukhsh MA, Jafry WS, Khansa RM, Gorski DH
Breast cancer research and treatment 2016 Jun;157(2):217-228
Breast cancer research and treatment 2016 Jun;157(2):217-228
Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation.
Bergström P, Agholme L, Nazir FH, Satir TM, Toombs J, Wellington H, Strandberg J, Bontell TO, Kvartsberg H, Holmström M, Boreström C, Simonsson S, Kunath T, Lindahl A, Blennow K, Hanse E, Portelius E, Wray S, Zetterberg H
Scientific reports 2016 Jul 7;6:29200
Scientific reports 2016 Jul 7;6:29200
Interleukin-17 potently increases non-small cell lung cancer growth.
Wei L, Wang H, Yang F, Ding Q, Zhao J
Molecular medicine reports 2016 Feb;13(2):1673-80
Molecular medicine reports 2016 Feb;13(2):1673-80
Cell stress increases ATP release in NLRP3 inflammasome-mediated autoinflammatory diseases, resulting in cytokine imbalance.
Carta S, Penco F, Lavieri R, Martini A, Dinarello CA, Gattorno M, Rubartelli A
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):2835-40
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):2835-40
Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates.
Cuellar TL, Barnes D, Nelson C, Tanguay J, Yu SF, Wen X, Scales SJ, Gesch J, Davis D, van Brabant Smith A, Leake D, Vandlen R, Siebel CW
Nucleic acids research 2015 Jan;43(2):1189-203
Nucleic acids research 2015 Jan;43(2):1189-203
Human mutations in methylenetetrahydrofolate dehydrogenase 1 impair nuclear de novo thymidylate biosynthesis.
Field MS, Kamynina E, Watkins D, Rosenblatt DS, Stover PJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 13;112(2):400-5
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 13;112(2):400-5
Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes.
Lin YS, Yang CC, Hsu CC, Hsu JT, Wu SC, Lin CJ, Cheng WT
Transgenic research 2015 Feb;24(1):61-71
Transgenic research 2015 Feb;24(1):61-71
Deubiquitinating enzymes regulate PARK2-mediated mitophagy.
Wang Y, Serricchio M, Jauregui M, Shanbhag R, Stoltz T, Di Paolo CT, Kim PK, McQuibban GA
Autophagy 2015 Apr 3;11(4):595-606
Autophagy 2015 Apr 3;11(4):595-606
Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer.
Speyer CL, Hachem AH, Assi AA, Johnson JS, DeVries JA, Gorski DH
PloS one 2014;9(3):e88830
PloS one 2014;9(3):e88830
Metabotropic glutamate receptor-1 contributes to progression in triple negative breast cancer.
Banda M, Speyer CL, Semma SN, Osuala KO, Kounalakis N, Torres Torres KE, Barnard NJ, Kim HJ, Sloane BF, Miller FR, Goydos JS, Gorski DH
PloS one 2014;9(1):e81126
PloS one 2014;9(1):e81126
Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress.
Venè R, Cardinali B, Arena G, Ferrari N, Benelli R, Minghelli S, Poggi A, Noonan DM, Albini A, Tosetti F
Neoplasia (New York, N.Y.) 2014 Sep;16(9):710-22
Neoplasia (New York, N.Y.) 2014 Sep;16(9):710-22
C10ORF10/DEPP, a transcriptional target of FOXO3, regulates ROS-sensitivity in human neuroblastoma.
Salcher S, Hagenbuchner J, Geiger K, Seiter MA, Rainer J, Kofler R, Hermann M, Kiechl-Kohlendorfer U, Ausserlechner MJ, Obexer P
Molecular cancer 2014 Sep 28;13:224
Molecular cancer 2014 Sep 28;13:224
Spreading of amyloid-β peptides via neuritic cell-to-cell transfer is dependent on insufficient cellular clearance.
Domert J, Rao SB, Agholme L, Brorsson AC, Marcusson J, Hallbeck M, Nath S
Neurobiology of disease 2014 May;65:82-92
Neurobiology of disease 2014 May;65:82-92
Cortical synaptic NMDA receptor deficits in α7 nicotinic acetylcholine receptor gene deletion models: implications for neuropsychiatric diseases.
Lin H, Hsu FC, Baumann BH, Coulter DA, Lynch DR
Neurobiology of disease 2014 Mar;63:129-40
Neurobiology of disease 2014 Mar;63:129-40
MicroRNA-340 as a modulator of RAS-RAF-MAPK signaling in melanoma.
Poenitzsch Strong AM, Setaluri V, Spiegelman VS
Archives of biochemistry and biophysics 2014 Dec 1;563:118-24
Archives of biochemistry and biophysics 2014 Dec 1;563:118-24
A unifying mechanism for cancer cell death through ion channel activation by HAMLET.
Storm P, Klausen TK, Trulsson M, Ho C S J, Dosnon M, Westergren T, Chao Y, Rydström A, Yang H, Pedersen SF, Svanborg C
PloS one 2013;8(3):e58578
PloS one 2013;8(3):e58578
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice.
Borghgraef P, Menuet C, Theunis C, Louis JV, Devijver H, Maurin H, Smet-Nocca C, Lippens G, Hilaire G, Gijsen H, Moechars D, Van Leuven F
PloS one 2013;8(12):e84442
PloS one 2013;8(12):e84442
Difference in protein expression profile and chemotherapy drugs response of different progression stages of LNCaP sublines and other human prostate cancer cells.
Lin HP, Lin CY, Hsiao PH, Wang HD, Jiang SS, Hsu JM, Jim WT, Chen M, Kung HJ, Chuu CP
PloS one 2013;8(12):e82625
PloS one 2013;8(12):e82625
Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress.
Wang YI, Bettaieb A, Sun C, DeVerse JS, Radecke CE, Mathew S, Edwards CM, Haj FG, Passerini AG, Simon SI
PloS one 2013;8(10):e78322
PloS one 2013;8(10):e78322
Evidence of epidermal growth factor receptor expression in uveal melanoma: inhibition of epidermal growth factor-mediated signalling by Gefitinib and Cetuximab triggered antibody-dependent cellular cytotoxicity.
Amaro A, Mirisola V, Angelini G, Musso A, Tosetti F, Esposito AI, Perri P, Lanza F, Nasciuti F, Mosci C, Puzone R, Salvi S, Truini M, Poggi A, Pfeffer U
European journal of cancer (Oxford, England : 1990) 2013 Oct;49(15):3353-65
European journal of cancer (Oxford, England : 1990) 2013 Oct;49(15):3353-65
Shear stress modulates VCAM-1 expression in response to TNF-α and dietary lipids via interferon regulatory factor-1 in cultured endothelium.
DeVerse JS, Sandhu AS, Mendoza N, Edwards CM, Sun C, Simon SI, Passerini AG
American journal of physiology. Heart and circulatory physiology 2013 Oct 15;305(8):H1149-57
American journal of physiology. Heart and circulatory physiology 2013 Oct 15;305(8):H1149-57
Modulation of airway epithelial cell functions by Pidotimod: NF-kB cytoplasmatic expression and its nuclear translocation are associated with an increased TLR-2 expression.
Carta S, Silvestri M, Rossi GA
Italian journal of pediatrics 2013 May 10;39:29
Italian journal of pediatrics 2013 May 10;39:29
Spinal serum-inducible and glucocorticoid-inducible kinase 1 mediates neuropathic pain via kalirin and downstream PSD-95-dependent NR2B phosphorylation in rats.
Peng HY, Chen GD, Lai CY, Hsieh MC, Lin TB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Mar 20;33(12):5227-40
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Mar 20;33(12):5227-40
Impact of CXCL1 overexpression on growth and invasion of prostate cancer cell.
Benelli R, Stigliani S, Minghelli S, Carlone S, Ferrari N
The Prostate 2013 Jun;73(9):941-51
The Prostate 2013 Jun;73(9):941-51
Celecoxib induces proliferation and Amphiregulin production in colon subepithelial myofibroblasts, activating erk1-2 signaling in synergy with EGFR.
Benelli R, Venè R, Minghelli S, Carlone S, Gatteschi B, Ferrari N
Cancer letters 2013 Jan 1;328(1):73-82
Cancer letters 2013 Jan 1;328(1):73-82
Exogenous SPARC suppresses proliferation and migration of prostate cancer by interacting with integrin β1.
Shin M, Mizokami A, Kim J, Ofude M, Konaka H, Kadono Y, Kitagawa Y, Miwa S, Kumaki M, Keller ET, Namiki M
The Prostate 2013 Aug;73(11):1159-70
The Prostate 2013 Aug;73(11):1159-70
Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers.
Loke WM, Lam KM, Chong WL, Chew SE, Quek AM, Lim ECh, Seet RC
Free radical research 2012 Oct;46(10):1230-7
Free radical research 2012 Oct;46(10):1230-7
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
Innate immunity 2012 Jun;18(3):531-40
Innate immunity 2012 Jun;18(3):531-40
Shear stress modulates RAGE-mediated inflammation in a model of diabetes-induced metabolic stress.
DeVerse JS, Bailey KA, Jackson KN, Passerini AG
American journal of physiology. Heart and circulatory physiology 2012 Jun 15;302(12):H2498-508
American journal of physiology. Heart and circulatory physiology 2012 Jun 15;302(12):H2498-508
Regulation of neuroendocrine differentiation by AKT/hnRNPK/AR/β-catenin signaling in prostate cancer cells.
Ciarlo M, Benelli R, Barbieri O, Minghelli S, Barboro P, Balbi C, Ferrari N
International journal of cancer 2012 Aug 1;131(3):582-90
International journal of cancer 2012 Aug 1;131(3):582-90
Upregulation of cellular Bcl-2 by the KSHV encoded RTA promotes virion production.
Gao J, Cai Q, Lu J, Jha HC, Robertson ES
PloS one 2011;6(8):e23892
PloS one 2011;6(8):e23892
Mthfs is an Essential Gene in Mice and a Component of the Purinosome.
Field MS, Anderson DD, Stover PJ
Frontiers in genetics 2011;2:36
Frontiers in genetics 2011;2:36
Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF.
Johnen S, Kazanskaya O, Armogan N, Stickelmann C, Stöcker M, Walter P, Thumann G
Investigative ophthalmology & visual science 2011 Jul 23;52(8):5400-7
Investigative ophthalmology & visual science 2011 Jul 23;52(8):5400-7
Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells.
Cheng G, Shao Z, Chaudhari B, Agrawal DK
American journal of physiology. Lung cellular and molecular physiology 2007 Nov;293(5):L1339-47
American journal of physiology. Lung cellular and molecular physiology 2007 Nov;293(5):L1339-47
Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase.
Allen RW, Trach KA, Hoch JA
The Journal of biological chemistry 1987 Jan 15;262(2):649-53
The Journal of biological chemistry 1987 Jan 15;262(2):649-53
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
