Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 10-10011 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant GAPDH
protein (amino acids 120-320) was u
sed as the immunogen for this antib
ody.- Isotype
- IgG
- Antibody clone number
- ABM22C5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 0.5 µg/ml on (1) Panc-1, (2) EL-4, (3) Jurkat and (4) HepG2 lysates.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on (1) CHO-K1 and (2) CHO/PD1 transfected Lysates.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Lung, (2) h Testis, (3) h Liver and (4) h Ovary lysates.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on Raw cell Lysate.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Brain, (2) m Brain, (3) h Pancrease, (4) h Kidney and (5) m Skeletal Muscle lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GAPDH in human Liver tissue using GAPDH antibody (Clone: ABM22C5) at 3 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GAPDH in human Small intestine tissue using GAPDH antibody (Clone: ABM22C5) at 10 µg/ml.
- Protocol
- Protocol