Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab104788 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant GAPDH Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human GAPDH Antibody, Recombinant (RR688), could be used for WB, IHC, Flow, IF, ICC, IP and so on.Application:WB: 1/5000 - 1/40000 ÊÊÊÊÊÊIHC: 1/1600 - 1/3200Flow: 1/200 - 1/1000IF/ICC: 1/2000 - 1/10000IP: 1/50Protein FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.ÊPost-translationalS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 200-300- Antibody clone number
- RR688
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant GAPDH Antibody (Ab104788) - Western Blot All lanes: Recombinant GAPDH Antibody (Ab104788) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 1.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant GAPDH Antibody (Ab104788) - IF IF analysis of GAPDH (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant GAPDH Antibody (Ab104788) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant GAPDH Antibody (Ab104788) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of prostate cancer tissue labelling GAPDH with Recombinant GAPDH Antibody (Ab104788) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant GAPDH Antibody (Ab104788) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant GAPDH Antibody (Ab104788) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant GAPDH Antibody (Ab104788) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
