Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-47339 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47339, RRID:AB_10010294
- Product name
- Mouse Monoclonal GAPDH Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Androgen receptor dampens tissue factor expression via nuclear factor-κB and early growth response protein 1.
Peroxisome proliferator-activated receptor-γ inhibits pancreatic cancer cell invasion and metastasis via regulating MMP-2 expression through PTEN.
Nodal activates smad and extracellular signal-regulated kinases 1/2 pathways promoting renal cell carcinoma proliferation.
The Parkinson's-associated protein DJ-1 regulates the 20S proteasome.
Dynamic regulation of the COP9 signalosome in response to DNA damage.
Hoesel B, Mussbacher M, Dikorman B, Salzmann M, Assinger A, Hell L, Thaler J, Basílio J, Moser B, Resch U, Paar H, Mackman N, Schmid JA
Journal of thrombosis and haemostasis : JTH 2018 Apr;16(4):749-758
Journal of thrombosis and haemostasis : JTH 2018 Apr;16(4):749-758
Peroxisome proliferator-activated receptor-γ inhibits pancreatic cancer cell invasion and metastasis via regulating MMP-2 expression through PTEN.
Li Y, Zhang DW, Lin DQ, Cao LQ
Molecular medicine reports 2015 Oct;12(4):6255-60
Molecular medicine reports 2015 Oct;12(4):6255-60
Nodal activates smad and extracellular signal-regulated kinases 1/2 pathways promoting renal cell carcinoma proliferation.
Zhang Z, Jiang T, Li Q, Wang J, Yang D, Li X, Wang Q, Song X
Molecular medicine reports 2015 Jul;12(1):587-94
Molecular medicine reports 2015 Jul;12(1):587-94
The Parkinson's-associated protein DJ-1 regulates the 20S proteasome.
Moscovitz O, Ben-Nissan G, Fainer I, Pollack D, Mizrachi L, Sharon M
Nature communications 2015 Apr 2;6:6609
Nature communications 2015 Apr 2;6:6609
Dynamic regulation of the COP9 signalosome in response to DNA damage.
Füzesi-Levi MG, Ben-Nissan G, Bianchi E, Zhou H, Deery MJ, Lilley KS, Levin Y, Sharon M
Molecular and cellular biology 2014 Mar;34(6):1066-76
Molecular and cellular biology 2014 Mar;34(6):1066-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.