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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F50850-0.4ML - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GAPDH Antibody
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml, 0.08 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using GAPDH antibody; Ab was diluted at 1:1000 for each lane. Predicted molecular weight ~36kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH antibody and 1) A2058, 2) A375, and 3) CEM lysate. Predicted molecular weight ~36kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human prostate carcinoma with GAPDH antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using GAPDH antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
