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- Antigen structure
- References [18]
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- Western blot [6]
- Immunohistochemistry [4]
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- Product number
- NB300-322 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-322, RRID:AB_10001458
- Product name
- Rabbit Polyclonal GAPDH Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Pathogenesis and pathophysiology of autosomal dominant sleep-related hypermotor epilepsy with S284L-mutant α4 subunit of nicotinic ACh receptor.
Upregulated and Hyperactivated Thalamic Connexin 43 Plays Important Roles in Pathomechanisms of Cognitive Impairment and Seizure of Autosomal Dominant Sleep-Related Hypermotor Epilepsy with S284L-Mutant α4 Subunit of Nicotinic ACh Receptor.
Upregulated Connexin 43 Induced by Loss-of-Functional S284L-Mutant α4 Subunit of Nicotinic ACh Receptor Contributes to Pathomechanisms of Autosomal Dominant Sleep-Related Hypermotor Epilepsy.
Activation of Astroglial Connexin is Involved in Concentration-Dependent Double-Edged Sword Clinical Action of Clozapine.
An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis.
Cold shock protein YB-1 is involved in hypoxia-dependent gene transcription.
Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system.
Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy.
Gem-induced cytoskeleton remodeling increases cellular migration of HTLV-1-infected cells, formation of infected-to-target T-cell conjugates and viral transmission.
Function of RasGRP3 in the formation and progression of human breast cancer.
Human P301L-mutant tau expression in mouse entorhinal-hippocampal network causes tau aggregation and presynaptic pathology but no cognitive deficits.
Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells.
Mammary epithelial-specific disruption of c-Src impairs cell cycle progression and tumorigenesis.
Transcription factor Stat5 knockdown enhances androgen receptor degradation and delays castration-resistant prostate cancer progression in vivo.
Induction of filopodia-like protrusions by transmembrane agrin: role of agrin glycosaminoglycan chains and Rho-family GTPases.
Repression of BIRC5/survivin by FOXO3/FKHRL1 sensitizes human neuroblastoma cells to DNA damage-induced apoptosis.
The role of community organizations in AIDS prevention in two Latino communities in New York City.
Fukuyama K, Fukuzawa M, Shiroyama T, Okada M
British journal of pharmacology 2020 May;177(9):2143-2162
British journal of pharmacology 2020 May;177(9):2143-2162
Upregulated and Hyperactivated Thalamic Connexin 43 Plays Important Roles in Pathomechanisms of Cognitive Impairment and Seizure of Autosomal Dominant Sleep-Related Hypermotor Epilepsy with S284L-Mutant α4 Subunit of Nicotinic ACh Receptor.
Fukuyama K, Fukuzawa M, Okada M
Pharmaceuticals (Basel, Switzerland) 2020 May 18;13(5)
Pharmaceuticals (Basel, Switzerland) 2020 May 18;13(5)
Upregulated Connexin 43 Induced by Loss-of-Functional S284L-Mutant α4 Subunit of Nicotinic ACh Receptor Contributes to Pathomechanisms of Autosomal Dominant Sleep-Related Hypermotor Epilepsy.
Fukuyama K, Fukuzawa M, Okubo R, Okada M
Pharmaceuticals (Basel, Switzerland) 2020 Mar 29;13(4)
Pharmaceuticals (Basel, Switzerland) 2020 Mar 29;13(4)
Activation of Astroglial Connexin is Involved in Concentration-Dependent Double-Edged Sword Clinical Action of Clozapine.
Fukuyama K, Okubo R, Murata M, Shiroyama T, Okada M
Cells 2020 Feb 11;9(2)
Cells 2020 Feb 11;9(2)
An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis.
Smith HW, Hirukawa A, Sanguin-Gendreau V, Nandi I, Dufour CR, Zuo D, Tandoc K, Leibovitch M, Singh S, Rennhack JP, Swiatnicki M, Lavoie C, Papavasiliou V, Temps C, Carragher NO, Unciti-Broceta A, Savage P, Basik M, van Hoef V, Larsson O, Cooper CL, Vargas Calderon AC, Beith J, Millar E, Selinger C, Giguère V, Park M, Harris LN, Varadan V, Andrechek ER, O'Toole SA, Topisirovic I, Muller WJ
Nature communications 2019 Jul 1;10(1):2901
Nature communications 2019 Jul 1;10(1):2901
Cold shock protein YB-1 is involved in hypoxia-dependent gene transcription.
Rauen T, Frye BC, Wang J, Raffetseder U, Alidousty C, En-Nia A, Floege J, Mertens PR
Biochemical and biophysical research communications 2016 Sep 16;478(2):982-7
Biochemical and biophysical research communications 2016 Sep 16;478(2):982-7
Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system.
Duprey-Díaz MV, Blagburn JM, Blanco RE
Journal of chemical neuroanatomy 2016 Nov;77:80-92
Journal of chemical neuroanatomy 2016 Nov;77:80-92
Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy.
Wu JH, Liu JH, Ko YC, Wang CT, Chung YC, Chu KC, Liu TT, Chao HM, Jiang YJ, Chen SJ, Chung MY
Human molecular genetics 2016 Apr 15;25(8):1637-47
Human molecular genetics 2016 Apr 15;25(8):1637-47
Gem-induced cytoskeleton remodeling increases cellular migration of HTLV-1-infected cells, formation of infected-to-target T-cell conjugates and viral transmission.
Chevalier SA, Turpin J, Cachat A, Afonso PV, Gessain A, Brady JN, Pise-Masison CA, Mahieux R
PLoS pathogens 2014 Feb;10(2):e1003917
PLoS pathogens 2014 Feb;10(2):e1003917
Function of RasGRP3 in the formation and progression of human breast cancer.
Nagy Z, Kovács I, Török M, Tóth D, Vereb G, Buzás K, Juhász I, Blumberg PM, Bíró T, Czifra G
Molecular cancer 2014 Apr 29;13:96
Molecular cancer 2014 Apr 29;13:96
Human P301L-mutant tau expression in mouse entorhinal-hippocampal network causes tau aggregation and presynaptic pathology but no cognitive deficits.
Harris JA, Koyama A, Maeda S, Ho K, Devidze N, Dubal DB, Yu GQ, Masliah E, Mucke L
PloS one 2012;7(9):e45881
PloS one 2012;7(9):e45881
Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
Huang Y, Bell LN, Okamura J, Kim MS, Mohney RP, Guerrero-Preston R, Ratovitski EA
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
Reciprocal regulation controlling the expression of CPI-17, a specific inhibitor protein for the myosin light chain phosphatase in vascular smooth muscle cells.
Kim JI, Urban M, Young GD, Eto M
American journal of physiology. Cell physiology 2012 Jul 1;303(1):C58-68
American journal of physiology. Cell physiology 2012 Jul 1;303(1):C58-68
Mammary epithelial-specific disruption of c-Src impairs cell cycle progression and tumorigenesis.
Marcotte R, Smith HW, Sanguin-Gendreau V, McDonough RV, Muller WJ
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):2808-13
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 21;109(8):2808-13
Transcription factor Stat5 knockdown enhances androgen receptor degradation and delays castration-resistant prostate cancer progression in vivo.
Thomas C, Zoubeidi A, Kuruma H, Fazli L, Lamoureux F, Beraldi E, Monia BP, MacLeod AR, Thüroff JW, Gleave ME
Molecular cancer therapeutics 2011 Feb;10(2):347-59
Molecular cancer therapeutics 2011 Feb;10(2):347-59
Induction of filopodia-like protrusions by transmembrane agrin: role of agrin glycosaminoglycan chains and Rho-family GTPases.
Lin L, McCroskery S, Ross JM, Chak Y, Neuhuber B, Daniels MP
Experimental cell research 2010 Aug 15;316(14):2260-77
Experimental cell research 2010 Aug 15;316(14):2260-77
Repression of BIRC5/survivin by FOXO3/FKHRL1 sensitizes human neuroblastoma cells to DNA damage-induced apoptosis.
Obexer P, Hagenbuchner J, Unterkircher T, Sachsenmaier N, Seifarth C, Böck G, Porto V, Geiger K, Ausserlechner M
Molecular biology of the cell 2009 Apr;20(7):2041-8
Molecular biology of the cell 2009 Apr;20(7):2041-8
The role of community organizations in AIDS prevention in two Latino communities in New York City.
Freudenberg N, Trinidad U
Health education quarterly 1992 Summer;19(2):219-32
Health education quarterly 1992 Summer;19(2):219-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of mouse squamous cell carcinoma using NB300-322 at a dilution of 1:200.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of human lung carcinoma using NB300-322 at a dilution of 1:200.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB
