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- Antibody Data
- Antigen structure
- References [49]
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- Validations
- Western blot [8]
- Immunohistochemistry [5]
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- Product number
- NB300-320 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-320, RRID:AB_10001796
- Product name
- Goat Polyclonal GAPDH Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat, Canine, Drosophila
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies.
Molecular Regulation of the RhoGAP GRAF3 and Its Capacity to Limit Blood Pressure In Vivo.
Hysteretic Genetic Circuit for Detection of Proteasomal Degradation in Mammalian Cells.
Selective androgen receptor modulator, S42 has anabolic and anti-catabolic effects on cultured myotubes.
Sialylation of MUC4β N-glycans by ST6GAL1 orchestrates human airway epithelial cell differentiation associated with type-2 inflammation.
Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity.
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation.
Dysfunctional ErbB2, an EGF receptor family member, hinders repair of airway epithelial cells from asthmatic patients.
Acute Enhancement of Cardiac Function by Phosphodiesterase Type 1 Inhibition.
Lipolysis Triggers a Systemic Insulin Response Essential for Efficient Energy Replenishment of Activated Brown Adipose Tissue in Mice.
JNK is antagonized to ensure the correct number of interommatidial cells pattern the Drosophila retina.
IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells.
A scar-like lesion is apparent in basement membrane after wound repair in vivo.
Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons.
Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells.
Systemic Correction of Murine Glycogen Storage Disease Type IV by an AAV-Mediated Gene Therapy.
Glial overexpression of Dube3a causes seizures and synaptic impairments in Drosophila concomitant with down regulation of the Na+/K+ pump ATPα.
A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction.
LSD1 modulates stress-evoked transcription of immediate early genes and emotional behavior.
Prenatal Exposure to Bisphenol A Disrupts Mouse Fetal Liver Maturation in a Sex-Specific Manner.
Targeting glutamate uptake to treat alcohol use disorders.
LSD1 Neurospecific Alternative Splicing Controls Neuronal Excitability in Mouse Models of Epilepsy.
Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes.
Characterization of neurons from immortalized dental pulp stem cells for the study of neurogenetic disorders.
Spatial profiles of markers of glycolysis, mitochondria, and proton pumps in a rat glioma suggest coordinated programming for proliferation.
The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
Prenatal exposure to bisphenol A disrupts mouse fetal lung development.
Kindlin-2 tyrosine phosphorylation and interaction with Src serve as a regulatable switch in the integrin outside-in signaling circuit.
Correction of glycogen storage disease type III with rapamycin in a canine model.
Dystrophin is a tumor suppressor in human cancers with myogenic programs.
Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control.
Probing the stiffness of isolated nucleoli by atomic force microscopy.
The Microtubule Regulatory Protein Stathmin Is Required to Maintain the Integrity of Axonal Microtubules in Drosophila.
Stbd1 is highly elevated in skeletal muscle of Pompe disease mice but suppression of its expression does not affect lysosomal glycogen accumulation.
BCLAF1 is a radiation-induced H2AX-interacting partner involved in γH2AX-mediated regulation of apoptosis and DNA repair.
Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption.
RNAi-mediated knockdown of α-enolase increases the sensitivity of tumor cells to antitubulin chemotherapeutics.
Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection.
Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse.
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.
Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.
Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.
Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.
Krohn L, Öztürk TN, Vanderperre B, Ouled Amar Bencheikh B, Ruskey JA, Laurent SB, Spiegelman D, Postuma RB, Arnulf I, Hu MTM, Dauvilliers Y, Högl B, Stefani A, Monaca CC, Plazzi G, Antelmi E, Ferini-Strambi L, Heidbreder A, Rudakou U, Cochen De Cock V, Young P, Wolf P, Oliva P, Zhang XK, Greenbaum L, Liong C, Gagnon JF, Desautels A, Hassin-Baer S, Montplaisir JY, Dupré N, Rouleau GA, Fon EA, Trempe JF, Lamoureux G, Alcalay RN, Gan-Or Z
Annals of neurology 2020 Jan;87(1):139-153
Annals of neurology 2020 Jan;87(1):139-153
Molecular Regulation of the RhoGAP GRAF3 and Its Capacity to Limit Blood Pressure In Vivo.
Dee RA, Bai X, Mack CP, Taylor JM
Cells 2020 Apr 22;9(4)
Cells 2020 Apr 22;9(4)
Hysteretic Genetic Circuit for Detection of Proteasomal Degradation in Mammalian Cells.
Zeng Y, Bhagyashree B, Zhao W, Nguyen T, Segatori L
ACS synthetic biology 2019 Sep 20;8(9):2025-2035
ACS synthetic biology 2019 Sep 20;8(9):2025-2035
Selective androgen receptor modulator, S42 has anabolic and anti-catabolic effects on cultured myotubes.
Muta Y, Tanaka T, Hamaguchi Y, Hamanoue N, Motonaga R, Tanabe M, Nomiyama T, Nawata H, Yanase T
Biochemistry and biophysics reports 2019 Mar;17:177-181
Biochemistry and biophysics reports 2019 Mar;17:177-181
Sialylation of MUC4β N-glycans by ST6GAL1 orchestrates human airway epithelial cell differentiation associated with type-2 inflammation.
Zhou X, Kinlough CL, Hughey RP, Jin M, Inoue H, Etling E, Modena BD, Kaminski N, Bleecker ER, Meyers DA, Jarjour NN, Trudeau JB, Holguin F, Ray A, Wenzel SE
JCI insight 2019 Mar 7;4(5)
JCI insight 2019 Mar 7;4(5)
Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity.
Patrón LA, Nagatomo K, Eves DT, Imad M, Young K, Torvund M, Guo X, Rogers GC, Zinsmaier KE
The Journal of cell biology 2019 Mar 4;218(3):993-1010
The Journal of cell biology 2019 Mar 4;218(3):993-1010
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation.
DeAngelis MW, Johnson RI
Journal of visualized experiments : JoVE 2019 Mar 15;(145)
Journal of visualized experiments : JoVE 2019 Mar 15;(145)
Dysfunctional ErbB2, an EGF receptor family member, hinders repair of airway epithelial cells from asthmatic patients.
Inoue H, Hattori T, Zhou X, Etling EB, Modena BD, Trudeau JB, Holguin F, Wenzel SE
The Journal of allergy and clinical immunology 2019 Jun;143(6):2075-2085.e10
The Journal of allergy and clinical immunology 2019 Jun;143(6):2075-2085.e10
Acute Enhancement of Cardiac Function by Phosphodiesterase Type 1 Inhibition.
Hashimoto T, Kim GE, Tunin RS, Adesiyun T, Hsu S, Nakagawa R, Zhu G, O'Brien JJ, Hendrick JP, Davis RE, Yao W, Beard D, Hoxie HR, Wennogle LP, Lee DI, Kass DA
Circulation 2018 Oct 30;138(18):1974-1987
Circulation 2018 Oct 30;138(18):1974-1987
Lipolysis Triggers a Systemic Insulin Response Essential for Efficient Energy Replenishment of Activated Brown Adipose Tissue in Mice.
Heine M, Fischer AW, Schlein C, Jung C, Straub LG, Gottschling K, Mangels N, Yuan Y, Nilsson SK, Liebscher G, Chen O, Schreiber R, Zechner R, Scheja L, Heeren J
Cell metabolism 2018 Oct 2;28(4):644-655.e4
Cell metabolism 2018 Oct 2;28(4):644-655.e4
JNK is antagonized to ensure the correct number of interommatidial cells pattern the Drosophila retina.
Bushnell HL, Feiler CE, Ketosugbo KF, Hellerman MB, Nazzaro VL, Johnson RI
Developmental biology 2018 Jan 1;433(1):94-107
Developmental biology 2018 Jan 1;433(1):94-107
IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells.
Hain A, Krämer M, Linka RM, Nakhaei-Rad S, Ahmadian MR, Häussinger D, Borkhardt A, Münk C
Scientific reports 2018 Feb 16;8(1):3217
Scientific reports 2018 Feb 16;8(1):3217
A scar-like lesion is apparent in basement membrane after wound repair in vivo.
Ramos-Lewis W, LaFever KS, Page-McCaw A
Matrix biology : journal of the International Society for Matrix Biology 2018 Dec;74:101-120
Matrix biology : journal of the International Society for Matrix Biology 2018 Dec;74:101-120
Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons.
Sullivan CS, Gotthard I, Wyatt EV, Bongu S, Mohan V, Weinberg RJ, Maness PF
Scientific reports 2018 Apr 18;8(1):6143
Scientific reports 2018 Apr 18;8(1):6143
Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells.
Grosse S, Penaud-Budloo M, Herrmann AK, Börner K, Fakhiri J, Laketa V, Krämer C, Wiedtke E, Gunkel M, Ménard L, Ayuso E, Grimm D
Journal of virology 2017 Oct 15;91(20)
Journal of virology 2017 Oct 15;91(20)
Systemic Correction of Murine Glycogen Storage Disease Type IV by an AAV-Mediated Gene Therapy.
Yi H, Zhang Q, Brooks ED, Yang C, Thurberg BL, Kishnani PS, Sun B
Human gene therapy 2017 Mar;28(3):286-294
Human gene therapy 2017 Mar;28(3):286-294
Glial overexpression of Dube3a causes seizures and synaptic impairments in Drosophila concomitant with down regulation of the Na+/K+ pump ATPα.
Hope KA, LeDoux MS, Reiter LT
Neurobiology of disease 2017 Dec;108:238-248
Neurobiology of disease 2017 Dec;108:238-248
A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction.
Bell RL, Hauser S, Rodd ZA, Liang T, Sari Y, McClintick J, Rahman S, Engleman EA
International review of neurobiology 2016;126:179-261
International review of neurobiology 2016;126:179-261
LSD1 modulates stress-evoked transcription of immediate early genes and emotional behavior.
Rusconi F, Grillo B, Ponzoni L, Bassani S, Toffolo E, Paganini L, Mallei A, Braida D, Passafaro M, Popoli M, Sala M, Battaglioli E
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 29;113(13):3651-6
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 29;113(13):3651-6
Prenatal Exposure to Bisphenol A Disrupts Mouse Fetal Liver Maturation in a Sex-Specific Manner.
DeBenedictis B, Guan H, Yang K
Journal of cellular biochemistry 2016 Feb;117(2):344-50
Journal of cellular biochemistry 2016 Feb;117(2):344-50
Targeting glutamate uptake to treat alcohol use disorders.
Rao PS, Bell RL, Engleman EA, Sari Y
Frontiers in neuroscience 2015;9:144
Frontiers in neuroscience 2015;9:144
LSD1 Neurospecific Alternative Splicing Controls Neuronal Excitability in Mouse Models of Epilepsy.
Rusconi F, Paganini L, Braida D, Ponzoni L, Toffolo E, Maroli A, Landsberger N, Bedogni F, Turco E, Pattini L, Altruda F, De Biasi S, Sala M, Battaglioli E
Cerebral cortex (New York, N.Y. : 1991) 2015 Sep;25(9):2729-40
Cerebral cortex (New York, N.Y. : 1991) 2015 Sep;25(9):2729-40
Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes.
Bader CA, Shandala T, Ng YS, Johnson IR, Brooks DA
Biology open 2015 Sep 9;4(11):1345-55
Biology open 2015 Sep 9;4(11):1345-55
Characterization of neurons from immortalized dental pulp stem cells for the study of neurogenetic disorders.
Urraca N, Memon R, El-Iyachi I, Goorha S, Valdez C, Tran QT, Scroggs R, Miranda-Carboni GA, Donaldson M, Bridges D, Reiter LT
Stem cell research 2015 Nov;15(3):722-730
Stem cell research 2015 Nov;15(3):722-730
Spatial profiles of markers of glycolysis, mitochondria, and proton pumps in a rat glioma suggest coordinated programming for proliferation.
Grillon E, Farion R, Reuveni M, Glidle A, Rémy C, Coles JA
BMC research notes 2015 Jun 2;8:207
BMC research notes 2015 Jun 2;8:207
The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
Obis T, Besalduch N, Hurtado E, Nadal L, Santafe MM, Garcia N, Tomàs M, Priego M, Lanuza MA, Tomàs J
Molecular brain 2015 Feb 10;8:8
Molecular brain 2015 Feb 10;8:8
Prenatal exposure to bisphenol A disrupts mouse fetal lung development.
Hijazi A, Guan H, Cernea M, Yang K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Dec;29(12):4968-77
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Dec;29(12):4968-77
Kindlin-2 tyrosine phosphorylation and interaction with Src serve as a regulatable switch in the integrin outside-in signaling circuit.
Qu H, Tu Y, Guan JL, Xiao G, Wu C
The Journal of biological chemistry 2014 Nov 7;289(45):31001-13
The Journal of biological chemistry 2014 Nov 7;289(45):31001-13
Correction of glycogen storage disease type III with rapamycin in a canine model.
Yi H, Brooks ED, Thurberg BL, Fyfe JC, Kishnani PS, Sun B
Journal of molecular medicine (Berlin, Germany) 2014 Jun;92(6):641-50
Journal of molecular medicine (Berlin, Germany) 2014 Jun;92(6):641-50
Dystrophin is a tumor suppressor in human cancers with myogenic programs.
Wang Y, Marino-Enriquez A, Bennett RR, Zhu M, Shen Y, Eilers G, Lee JC, Henze J, Fletcher BS, Gu Z, Fox EA, Antonescu CR, Fletcher CD, Guo X, Raut CP, Demetri GD, van de Rijn M, Ordog T, Kunkel LM, Fletcher JA
Nature genetics 2014 Jun;46(6):601-6
Nature genetics 2014 Jun;46(6):601-6
Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control.
McLelland GL, Soubannier V, Chen CX, McBride HM, Fon EA
The EMBO journal 2014 Feb 18;33(4):282-95
The EMBO journal 2014 Feb 18;33(4):282-95
Probing the stiffness of isolated nucleoli by atomic force microscopy.
Louvet E, Yoshida A, Kumeta M, Takeyasu K
Histochemistry and cell biology 2014 Apr;141(4):365-81
Histochemistry and cell biology 2014 Apr;141(4):365-81
The Microtubule Regulatory Protein Stathmin Is Required to Maintain the Integrity of Axonal Microtubules in Drosophila.
Duncan JE, Lytle NK, Zuniga A, Goldstein LS
PloS one 2013;8(6):e68324
PloS one 2013;8(6):e68324
Stbd1 is highly elevated in skeletal muscle of Pompe disease mice but suppression of its expression does not affect lysosomal glycogen accumulation.
Yi H, Fredrickson KB, Das S, Kishnani PS, Sun B
Molecular genetics and metabolism 2013 Jul;109(3):312-4
Molecular genetics and metabolism 2013 Jul;109(3):312-4
BCLAF1 is a radiation-induced H2AX-interacting partner involved in γH2AX-mediated regulation of apoptosis and DNA repair.
Lee YY, Yu YB, Gunawardena HP, Xie L, Chen X
Cell death & disease 2012 Jul 26;3:e359
Cell death & disease 2012 Jul 26;3:e359
Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
Noelker C, Schwake M, Balzer-Geldsetzer M, Bacher M, Popp J, Schlegel J, Eggert K, Oertel WH, Klockgether T, Dodel RC
Neuroscience letters 2012 Jan 17;507(1):10-5
Neuroscience letters 2012 Jan 17;507(1):10-5
FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption.
Nechipurenko IV, Broihier HT
The Journal of cell biology 2012 Feb 6;196(3):345-62
The Journal of cell biology 2012 Feb 6;196(3):345-62
RNAi-mediated knockdown of α-enolase increases the sensitivity of tumor cells to antitubulin chemotherapeutics.
Georges E, Bonneau AM, Prinos P
International journal of biochemistry and molecular biology 2011;2(4):303-8
International journal of biochemistry and molecular biology 2011;2(4):303-8
Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection.
Weile C, Josefsen K, Buschard K
Clinical and experimental immunology 2011 Nov;166(2):251-7
Clinical and experimental immunology 2011 Nov;166(2):251-7
Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
Pulinilkunnil T, He H, Kong D, Asakura K, Peroni OD, Lee A, Kahn BB
The Journal of biological chemistry 2011 Mar 18;286(11):8798-809
The Journal of biological chemistry 2011 Mar 18;286(11):8798-809
Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse.
Besalduch N, Tomàs M, Santafé MM, Garcia N, Tomàs J, Lanuza MA
The Journal of comparative neurology 2010 Jan 10;518(2):211-28
The Journal of comparative neurology 2010 Jan 10;518(2):211-28
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ
Molecular vision 2009;15:283-95
Molecular vision 2009;15:283-95
Distinct functions for the catalytic and hemopexin domains of a Drosophila matrix metalloproteinase.
Glasheen BM, Kabra AT, Page-McCaw A
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2659-64
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2659-64
Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.
Kiepe D, Van Der Pas A, Ciarmatori S, Ständker L, Schütt B, Hoeflich A, Hügel U, Oh J, Tönshoff B
Endocrinology 2008 Oct;149(10):4901-11
Endocrinology 2008 Oct;149(10):4901-11
Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.
Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD
Cerebral cortex (New York, N.Y. : 1991) 2008 Nov;18(11):2560-73
Cerebral cortex (New York, N.Y. : 1991) 2008 Nov;18(11):2560-73
Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.
Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF
PloS one 2008 Feb 20;3(2):e1631
PloS one 2008 Feb 20;3(2):e1631
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
Nguyen M, Pace AJ, Koller BH
The Journal of experimental medicine 2007 Jun 11;204(6):1383-93
The Journal of experimental medicine 2007 Jun 11;204(6):1383-93
Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.
Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM, Strittmatter WJ
Nature medicine 1996 Mar;2(3):347-50
Nature medicine 1996 Mar;2(3):347-50
Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.
Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM, Strittmatter WJ
Nature medicine 1996 Mar;2(3):347-50
Nature medicine 1996 Mar;2(3):347-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - Staining of lysates of cell line HEK293 (A) and HeLa (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - Human Liver (A) and Tonsil (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - Staining of mouse liver (A) and rat brain (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - GAPDH theoretical molecular weight: 36 kDa. Staining of cell lines HEK293 (A) and HeLa (B) and Human Liver (C) and Tonsil (D) lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - GAPDH theoretical molecular weight: 36 kDa. Antibody (0.01 ug/mL) staining of Mouse Spleen (A), (0.1 ug/mL) Rat Brain (B) and (0.5 ug/mL) Rat Spleen (C) (35 ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - Staining of Human Liver (A) and Tonsil (B) with antibody at 0.001 ug/mL and (0.3ug/ml) Rat Brain lysate (C) with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - Staining of HEK293 (A) and HeLa (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.001 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-320] - stai Mutants Have Reduced Levels of alpha-Tubulin, Acetylated alpha-Tubulin, and Kinesin Heavy Chain Protein. Representative western blots of total protein extracted from stai mutant third instar larvae. Heterozygous stai/+ mutant larvae exhibit mild reductions in the levels of alpha-tubulin and acetylated alpha-tubulin compared with wild type larvae. Homozygous stai mutant larvae have dramatic reductions in the levels of alpha-tubulin, acetylated alpha-tubulin and the heavy chain subunit of the microtubule motor protein kinesin compared to wildtype. Df(2L) BSC239 represents a second chromosomal deficiency that excludes the stai gene. GAPDH is used as a loading control. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0068324), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Staining of paraffin embedded Human Kidney. Antibody at 2.5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-320] - Upregulation of GAPDH and Tom20 in C6 gliomas. A small glioma labeled with antibodies against GAPDH. The brightness and contrast have been increased. Image collected and cropped by CiteAb from the following publication (http://www.biomedcentral.com/1756-0500/8/207), licensed under a CC-BY licence.
