Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP16445PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP16445PU-N, RRID:AB_1928404
- Product name
- anti GAPDH
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region of the protein sequence according to NP_002037.2.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
Submitted references The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide.
A-kinase anchoring in dendritic cells is required for antigen presentation.
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.
Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation.
Carr AJ, Vugler AA, Yu L, Semo M, Coffey P, Moss SE, Greenwood J
Molecular vision 2011;17:1701-15
Molecular vision 2011;17:1701-15
A-kinase anchoring in dendritic cells is required for antigen presentation.
Schillace RV, Miller CL, Pisenti N, Grotzke JE, Swarbrick GM, Lewinsohn DM, Carr DW
PloS one 2009;4(3):e4807
PloS one 2009;4(3):e4807
Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.
Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ
Molecular vision 2009;15:283-95
Molecular vision 2009;15:283-95
Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.
Chakir K, Daya SK, Aiba T, Tunin RS, Dimaano VL, Abraham TP, Jaques-Robinson KM, Lai EW, Pacak K, Zhu WZ, Xiao RP, Tomaselli GF, Kass DA
Circulation 2009 Mar 10;119(9):1231-40
Circulation 2009 Mar 10;119(9):1231-40
Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation.
Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P
Experimental neurology 2008 Dec;214(2):347-61
Experimental neurology 2008 Dec;214(2):347-61
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16445PU-N GAPDH antibody staining of Human Tonsil Lysate at 0.1 µg/ml (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16445PU-N GAPDH antibody staining of Rat Liver Lysate at 0.5 µg/ml (RIPA buffer, 35 µg total protein per lane). Primary incubation was overnight. Detected by alkaline phosphatase (NBT/BCIP). Data provided by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16445PU-N GAPDH antibody staining of Human Liver Lysate at 0.1 µg/ml (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using Chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16445PU-N GAPDH antibody staining of PFA-perfused cryosection of Rat Liver at 20 µg/ml. Antigen retrieval with citrate buffer pH 3 by microwave for 20min, Cy3 detection. Data obtained by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.