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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-328 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-328, RRID:AB_2247684
- Product name
- Anti-GDNF
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Mechanisms of impaired nephrogenesis with fetal growth restriction: altered renal transcription and growth factor expression.
Cellular localization of GDNF and its GFRalpha1/RET receptor complex in the developing pancreas of cat.
The major determinant of the heparin binding of glial cell-line-derived neurotrophic factor is near the N-terminus and is dispensable for receptor binding.
Adenovirus-Mediated Gene Transfer of Glial Cell Line-Derived Neurotrophic Factor Prevents Ischemic Brain Injury After Transient Middle Cerebral Artery Occlusion in Rats
Abdel-Hakeem AK, Henry TQ, Magee TR, Desai M, Ross MG, Mansano RZ, Torday JS, Nast CC
American journal of obstetrics and gynecology 2008 Sep;199(3):252.e1-7
American journal of obstetrics and gynecology 2008 Sep;199(3):252.e1-7
Cellular localization of GDNF and its GFRalpha1/RET receptor complex in the developing pancreas of cat.
Lucini C, Maruccio L, Facello B, Cocchia N, Tortora G, Castaldo L
Journal of anatomy 2008 Nov;213(5):565-72
Journal of anatomy 2008 Nov;213(5):565-72
The major determinant of the heparin binding of glial cell-line-derived neurotrophic factor is near the N-terminus and is dispensable for receptor binding.
Alfano I, Vora P, Mummery RS, Mulloy B, Rider CC
The Biochemical journal 2007 May 15;404(1):131-40
The Biochemical journal 2007 May 15;404(1):131-40
Adenovirus-Mediated Gene Transfer of Glial Cell Line-Derived Neurotrophic Factor Prevents Ischemic Brain Injury After Transient Middle Cerebral Artery Occlusion in Rats
Hisashi Kitagawa, Chihoko Sasaki, Kenichi Sakai, Atsushi Mori, Yasuhide Mitsumoto, Toyoki Mori, Yoshimosuke Fukuchi, Yasuhiro Setoguchi, Koji Abe
Journal of Cerebral Blood Flow & Metabolism 1999 Dec;19(12):1336-1344
Journal of Cerebral Blood Flow & Metabolism 1999 Dec;19(12):1336-1344
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 24
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
