Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA1-18359 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GDNF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Neurotrophic Factors Protect the Intestinal Barrier from Rotavirus Insult in Mice.
Hagbom M, De Faria FM, Winberg ME, Westerberg S, Nordgren J, Sharma S, Keita ÅV, Loitto V, Magnusson KE, Svensson L
mBio 2020 Jan 21;11(1)
mBio 2020 Jan 21;11(1)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIG 6 GDNF mRNA and protein levels were significantly higher in the duodenum of rotavirus-infected mice. (A) The duodenum of infected ( n = 8) and noninfected ( n = 7) mouse pups were collected at 16 h p.i., and the Gdnf and TATA-binding protein ( Tbp ) mRNA levels were quantified using qPCR. There was significant upregulation of Gdnf mRNA in the infected mice. Data are means +- SEM. * , P < 0.05, unpaired t test. (B) The duodenum of infected and noninfected mouse pups were collected at 16 h p.i., and the GDNF protein levels were measured using ELISA. The infected mice had significantly higher levels of GDNF protein than the noninfected controls. Four biopsy specimens from noninfected pups had GDNF protein concentrations below the ELISA detection limit. Data are medians with interquartile range. * , P < 0.05, Mann-Whitney test ( n = 5). (C and D) GDNF was present in the enterocytes of mouse small intestine, mainly in the middle and tip of the villi. Immunofluorescence staining shows GDNF (green, Alexa Fluor 488) in the ileal enterocytes of rotavirus-infected mouse pups (red, Alexa Fluor 594) at 16 h p.i. (C) and noninfected mouse pups (D). The tissue was counterstained with 4',6-diamidino-2-phenylindole (DAPI) nuclear stain.