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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- TA328657 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-GDNF
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-GDNF
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GDNF
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of recombinant human GDNF (hGDNF): Lanes 1, 4: 50 ng hGDNF. Lanes 2, 5: 10 ng hGDNF. Lanes 3, 4 ng hGDNF. Lanes 1-3: Anti-GDNF antibody , (1:1000). Lanes 4-5: Anti-GDNF antibody preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of GDNF in rat C6 glioma cells. Immunocytochemical staining of GDNF in rat C6 glioma cells. A. Paraformaldehyde-fixed and permeabilized rat C6 glioma cells were stained with Anti-GDNF antibody, (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as in (A).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of GDNF in rat dorsal root ganglion (DRG). Immunohistochemical staining of rat DRG longitudinal frozen section using Anti-GDNF antibody, (1:50), followed by Alexa 555-conjugated goat anti-rabbit antibody (red staining). Hoechst 33342 (blue) was used as a counterstain. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of GDNF in rat hippocampus. Immunohistochemical staining of rat hippocampus frozen sections with Anti-GDNF antibody, (1:100), (red). A. GDNF staining is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal arrows) and in some interneurons (vertical arrow). B. Calbindin D28k (green) appears in a subset of cells in the pyramidal layer. C. Merge of GDNF and calbindin demonstrates some co-localization in neurons. DAPI is used as the counterstain (blue).
- Validation comment
- IHC
