Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB212-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GDNF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GDNF in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) BDNF, rhCNTF, rhNeurturin, rh beta-NGF, rhNT-3, or rhNT-4.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Proliferation Induced by GDNF and Neutralization by Human GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by the Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDNF Monoclonal Antibody (Catalog # MAB212). The ND50 is typically 0.4-2 μg/mL.
