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- Antigen structure
- References [2]
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- Product number
- 500-P81-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GDNF Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF. Anti-Human GDNF-specific antibody was purified by affinity chromatography employing an immobilized hHuman GDNF matrix. Sandwich ELISA: To detect Human GDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GDNF (500-P81Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GDNF. Western Blot: To detect Human GDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis.
Apomorphine-induced activation of dopamine receptors modulates FGF-2 expression in astrocytic cultures and promotes survival of dopaminergic neurons.
Watanabe Y, Kazuki Y, Kazuki K, Ebiki M, Nakanishi M, Nakamura K, Yoshida Yamakawa M, Hosokawa H, Ohbayashi T, Oshimura M, Nakashima K
Molecular therapy. Nucleic acids 2015 Oct 6;4(10):e253
Molecular therapy. Nucleic acids 2015 Oct 6;4(10):e253
Apomorphine-induced activation of dopamine receptors modulates FGF-2 expression in astrocytic cultures and promotes survival of dopaminergic neurons.
Li A, Guo H, Luo X, Sheng J, Yang S, Yin Y, Zhou J, Zhou J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jun;20(8):1263-5
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jun;20(8):1263-5
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human GDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of GDNF Polyclonal Antibody (Product # 500-P81-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech GDNF Polyclonal Antibody, Biotin (Product # 500-P81BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GDNF.
