PA1465

antibody from Boster Biological Technology

Targeting: GDNF ATF1, ATF2, HFB1-GDNF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA1465

Provider

Boster Biological Technology

Product name

Anti-GDNF Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for GDNF detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GDNF information: Molecular Weight: 23720 MW; Subcellular Localization: Secreted ; Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota.

Cao J, Wang K, Li N, Zhang L, Qin L, He Y, Wang J, Qu C, Miao J
International journal of biological macromolecules 2023 Jan 31;226:1319-1331

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SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.

Liu T, Wang Y, Lu L, Liu Y
Journal of nanobiotechnology 2022 Mar 27;20(1):159

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Culture bovine prospermatogonia with 2i medium.

Cai H, Jiang Y, Zhang S, Cai NN, Zhu WQ, Yang R, Tang B, Li ZY, Zhang XM
Andrologia 2021 Jul;53(6):e14056

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The interplay of BDNF-TrkB with NMDA receptor in propofol-induced cognition dysfunction : Mechanism for the effects of propofol on cognitive function.

Zhou J, Wang F, Zhang J, Li J, Ma L, Dong T, Zhuang Z
BMC anesthesiology 2018 Apr 5;18(1):35

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The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro.

Liu H, Lv P, Wu H, Zhang K, Xu F, Zheng L, Zhao J
Evidence-based complementary and alternative medicine : eCAM 2017;2017:4673289

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Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells.

Zhang X, Shi K, Li Y, Zhang H, Hao J
Reproductive toxicology (Elmsford, N.Y.) 2014 Jun;45:87-93

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Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction.

Liu J, Wang X, Liu Y, Yang N, Xu J, Ren X
Neural regeneration research 2013 Aug 15;8(23):2190-7

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Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey.

Huang XC, Xu W, Jiang JY
Neuroscience bulletin 2008 Jun;24(3):150-4

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Anti-GDNF antibody, PA1465, Western blottingLane 1: Recombinant Human GDNF Protein 10ngLane 2: Recombinant Human GDNF Protein 5ngLane 3: Recombinant Human GDNF Protein 2.5ngLane 4: Recombinant Human GDNF Protein 1.25ng

Additional image