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- Antibody Data
- Antigen structure
- References [33]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-212-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat GDNF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and rat GDNF in direct ELISAs and Western blots.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P39905- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
Destabilizing Domains Enable Long-Term and Inert Regulation of GDNF Expression in the Brain.
CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease.
Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury.
Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane.
The noble gas xenon provides protection and trophic stimulation to midbrain dopamine neurons.
Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects.
β-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms.
Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair.
Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease.
A monoclonal antibody-GDNF fusion protein is not neuroprotective and is associated with proliferative pancreatic lesions in parkinsonian monkeys.
Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson's disease.
Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF.
Efficient gene therapy-based method for the delivery of therapeutics to primate cortex.
Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS.
SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
Identification of neuregulin as a factor required for formation of aligned spermatogonia.
Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats.
Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis.
Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat.
Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinson's disease.
Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus).
Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus).
Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice.
Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice.
Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood.
Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood.
Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Destabilizing Domains Enable Long-Term and Inert Regulation of GDNF Expression in the Brain.
Quintino L, Namislo A, Davidsson M, Breger LS, Kavanagh P, Avallone M, Elgstrand-Wettergren E, Isaksson C, Lundberg C
Molecular therapy. Methods & clinical development 2018 Dec 14;11:29-39
Molecular therapy. Methods & clinical development 2018 Dec 14;11:29-39
CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease.
Stott SR, Hayat S, Carnwath T, Garas S, Sleeman JP, Barker RA
PloS one 2017;12(2):e0171748
PloS one 2017;12(2):e0171748
Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury.
Nagashima K, Miwa T, Soumiya H, Ushiro D, Takeda-Kawaguchi T, Tamaoki N, Ishiguro S, Sato Y, Miyamoto K, Ohno T, Osawa M, Kunisada T, Shibata T, Tezuka KI, Furukawa S, Fukumitsu H
Scientific reports 2017 Oct 18;7(1):13500
Scientific reports 2017 Oct 18;7(1):13500
Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane.
Dong Y, Kanda A, Noda K, Saito W, Ishida S
Scientific reports 2017 Mar 9;7:44266
Scientific reports 2017 Mar 9;7:44266
The noble gas xenon provides protection and trophic stimulation to midbrain dopamine neurons.
Lavaur J, Le Nogue D, Lemaire M, Pype J, Farjot G, Hirsch EC, Michel PP
Journal of neurochemistry 2017 Jul;142(1):14-28
Journal of neurochemistry 2017 Jul;142(1):14-28
Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects.
Thomsen GM, Alkaslasi M, Vit JP, Lawless G, Godoy M, Gowing G, Shelest O, Svendsen CN
Gene therapy 2017 Apr;24(4):245-252
Gene therapy 2017 Apr;24(4):245-252
β-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms.
Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum ND, Bridgewater D
The American journal of pathology 2014 May;184(5):1395-410
The American journal of pathology 2014 May;184(5):1395-410
Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair.
Wang TY, Bruggeman KA, Sheean RK, Turner BJ, Nisbet DR, Parish CL
The Journal of biological chemistry 2014 May 23;289(21):15044-51
The Journal of biological chemistry 2014 May 23;289(21):15044-51
Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S
PloS one 2013;8(3):e56186
PloS one 2013;8(3):e56186
Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease.
Quintino L, Manfré G, Wettergren EE, Namislo A, Isaksson C, Lundberg C
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Dec;21(12):2169-80
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Dec;21(12):2169-80
A monoclonal antibody-GDNF fusion protein is not neuroprotective and is associated with proliferative pancreatic lesions in parkinsonian monkeys.
Ohshima-Hosoyama S, Simmons HA, Goecks N, Joers V, Swanson CR, Bondarenko V, Velotta R, Brunner K, Wood LD, Hruban RH, Emborg ME
PloS one 2012;7(6):e39036
PloS one 2012;7(6):e39036
Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson's disease.
Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Jun;19(6):1048-57
Molecular therapy : the journal of the American Society of Gene Therapy 2011 Jun;19(6):1048-57
Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
Neuropharmacology 2010 Jun;58(7):1114-21
Neuropharmacology 2010 Jun;58(7):1114-21
Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF.
Emborg ME, Moirano J, Raschke J, Bondarenko V, Zufferey R, Peng S, Ebert AD, Joers V, Roitberg B, Holden JE, Koprich J, Lipton J, Kordower JH, Aebischer P
Neurobiology of disease 2009 Nov;36(2):303-11
Neurobiology of disease 2009 Nov;36(2):303-11
Efficient gene therapy-based method for the delivery of therapeutics to primate cortex.
Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 17;106(7):2407-11
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 17;106(7):2407-11
Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS.
Suzuki M, McHugh J, Tork C, Shelley B, Hayes A, Bellantuono I, Aebischer P, Svendsen CN
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Dec;16(12):2002-10
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Dec;16(12):2002-10
SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP Jr
Development (Cambridge, England) 2007 Sep;134(18):3327-38
Development (Cambridge, England) 2007 Sep;134(18):3327-38
Identification of neuregulin as a factor required for formation of aligned spermatogonia.
Hamra FK, Chapman KM, Nguyen D, Garbers DL
The Journal of biological chemistry 2007 Jan 5;282(1):721-30
The Journal of biological chemistry 2007 Jan 5;282(1):721-30
Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
Stroke 2006 Sep;37(9):2361-7
Stroke 2006 Sep;37(9):2361-7
Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats.
Xu L, Yan J, Chen D, Welsh AM, Hazel T, Johe K, Hatfield G, Koliatsos VE
Transplantation 2006 Oct 15;82(7):865-75
Transplantation 2006 Oct 15;82(7):865-75
Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis.
Fouchécourt S, Godet M, Sabido O, Durand P
The Journal of endocrinology 2006 Jul;190(1):59-71
The Journal of endocrinology 2006 Jul;190(1):59-71
Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat.
Oo TF, Ries V, Cho J, Kholodilov N, Burke RE
The Journal of comparative neurology 2005 Mar 28;484(1):57-67
The Journal of comparative neurology 2005 Mar 28;484(1):57-67
Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinson's disease.
Benner EJ, Mosley RL, Destache CJ, Lewis TB, Jackson-Lewis V, Gorantla S, Nemachek C, Green SR, Przedborski S, Gendelman HE
Proceedings of the National Academy of Sciences of the United States of America 2004 Jun 22;101(25):9435-40
Proceedings of the National Academy of Sciences of the United States of America 2004 Jun 22;101(25):9435-40
Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
Esper RM, Loeb JA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 7;24(27):6218-27
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 7;24(27):6218-27
Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus).
Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE
Experimental neurology 2003 Nov;184(1):536-48
Experimental neurology 2003 Nov;184(1):536-48
Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus).
Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE
Experimental neurology 2003 Nov;184(1):536-48
Experimental neurology 2003 Nov;184(1):536-48
Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice.
Sun W, Oppenheim RW
Molecular and cellular neurosciences 2003 Dec;24(4):875-86
Molecular and cellular neurosciences 2003 Dec;24(4):875-86
Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice.
Sun W, Oppenheim RW
Molecular and cellular neurosciences 2003 Dec;24(4):875-86
Molecular and cellular neurosciences 2003 Dec;24(4):875-86
Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood.
Pina Serra M, Quartu M, Ambu R, Follesa P, Del Fiacco M
Brain research 2002 Feb 22;928(1-2):138-46
Brain research 2002 Feb 22;928(1-2):138-46
Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood.
Pina Serra M, Quartu M, Ambu R, Follesa P, Del Fiacco M
Brain research 2002 Feb 22;928(1-2):138-46
Brain research 2002 Feb 22;928(1-2):138-46
Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H
Journal of neurochemistry 2002 Aug;82(4):961-75
Journal of neurochemistry 2002 Aug;82(4):961-75
Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H
Journal of neurochemistry 2002 Aug;82(4):961-75
Journal of neurochemistry 2002 Aug;82(4):961-75
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GDNF and GFR alpha-1 in Rat Dorsal Root Ganglia. GDNF and GFR alpha-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFR alpha-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND50 is typically 0.25-1 μg/mL.
