Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-54318 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-54318, RRID:AB_11014712
- Product name
- Rabbit Polyclonal GRP78/HSPA5 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. Detects a protein with a mass of approx. 78kDa corresponding to Grp78.
- Reactivity
- Human, Mouse, Rat, Canine, Drosophila, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells.
Chu H, Chan CM, Zhang X, Wang Y, Yuan S, Zhou J, Au-Yeung RK, Sze KH, Yang D, Shuai H, Hou Y, Li C, Zhao X, Poon VK, Leung SP, Yeung ML, Yan J, Lu G, Jin DY, Gao GF, Chan JF, Yuen KY
The Journal of biological chemistry 2018 Jul 27;293(30):11709-11726
The Journal of biological chemistry 2018 Jul 27;293(30):11709-11726
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - analysis of Human Glucose deprived glia cell lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody . Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - (1ug/ml) Lane 1: Rec. hGrp78 (100ng); Lane 2, 3 and 4: 7.5ug of SKBR3 lysate (human), MDCK lysate (dog) and MEF lysate (mouse).