MA5-45279

antibody from Invitrogen Antibodies

Targeting: HSPA5 BiP, GRP78

Western blot

Antibody data

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Validation data

Product number: MA5-45279 - Provider product page
Provider: Invitrogen Antibodies
Product name: GRP78 Monoclonal Antibody (1H11-1H7), PE
Antibody type: Monoclonal
Antigen: Other
Description: 0.5 µg/mL of MA5-45279 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.|Detects approximately 78kDa.
Reactivity: Human, Mouse, Rat, Bovine, Hamster, Rabbit, Xenopus
Host: Mouse
Conjugate: Yellow dye
Isotype: IgG
Antibody clone number: 1H11-1H7
Vial size: 100 μg
Concentration: 1 mg/mL
Storage: 4°C

HSPA5 protein structure - MA5-45279 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with GRP78 monoclonal antibody (Product # MA5-45279) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with GRP78 monoclonal antibody (Product # MA5-45279) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with GRP78 monoclonal antibody (Product # MA5-45279) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with GRP78 monoclonal antibody (Product # MA5-45279) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 antibody. (C) Composite. Heat Shocked at 42°C for 1h.