Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-33165 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP C Recombinant Rabbit Monoclonal Antibody (9G1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9G1
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- -20°C or -80°C if preferred
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of hnRNP C using a hnRNP C Monoclonal antibody (Product # MA5-33165) at a concentration of 0.66 µg/mL. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated hnRNP C from Hela whole cell lysate using a hnRNP C monoclonal antibody (Product # MA5-33165). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (20µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of hnRNP C using a hnRNP C Monoclonal antibody (Product # MA5-33165) at a concentration of 0.66 µg/mL. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hnRNP C in Hela cells using a hnRNP C monoclonal antibody (Product # MA5-33165) at a dilution of 1:22. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP C in paraffin embedded human kidney tissue using a hnRNP C monoclonal antibody (Product # MA5-33165) at a dilution of 1:66.35. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of hnRNP C in MCF-7 cells using a monoclonal antibody (Product # MA5-33165) at a dilution of 1:50. The cells were fixed with 70% Ethyl alcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1:200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
