Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PAB6157 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6157, RRID:AB_1575292
- Product name
- GRB7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of GRB7.
- Antigen sequence
ELDLSPPHLSSSPE-C
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains.
Margolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A, Schlessinger J
Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1;89(19):8894-8
Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1;89(19):8894-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GRB7 polyclonal antibody (Cat # PAB6157) staining (2 ug/mL) of A-431 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing GRB7 and probed with GRB7 polyclonal antibody (Cat # PAB6157) (mock transfection in first lane), tested by Origene.