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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB18096 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18096, RRID:AB_10716175
- Product name
- HSP90AB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HSP90AB1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A nonredundant human protein chip for antibody screening and serum profiling.
Domain mapping studies reveal that the M domain of hsp90 serves as a molecular scaffold to regulate Akt-dependent phosphorylation of endothelial nitric oxide synthase and NO release.
Lueking A, Possling A, Huber O, Beveridge A, Horn M, Eickhoff H, Schuchardt J, Lehrach H, Cahill DJ
Molecular & cellular proteomics : MCP 2003 Dec;2(12):1342-9
Molecular & cellular proteomics : MCP 2003 Dec;2(12):1342-9
Domain mapping studies reveal that the M domain of hsp90 serves as a molecular scaffold to regulate Akt-dependent phosphorylation of endothelial nitric oxide synthase and NO release.
Fontana J, Fulton D, Chen Y, Fairchild TA, McCabe TJ, Fujita N, Tsuruo T, Sessa WC
Circulation research 2002 May 3;90(8):866-73
Circulation research 2002 May 3;90(8):866-73
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells treated with TNF-a (20 ng/mL, 30 min), using HSP90AB1 polyclonal antibody (Cat # PAB18096).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells, treated with TNF-a (20 nM, 15 mins), using HSP90AB1 polyclonal antibody (Cat # PAB18096).Peptide "+" means "peptide blocking".
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90AB1 polyclonal antibody (Cat # PAB18096).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
