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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
- Other assay [4]
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- Product number
- PA3-012-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSP90 beta Polyclonal Antibody, Alexa Fluor™ 488
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3012A488 detects heat shock protein 90 beta (HSP90) from human, monkey, mouse, and rat tissues and cells. This antibody does not detect HSP86. The PA3012A488 immunogen is a synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Heat shock protein 90α (HSP90α), a substrate and chaperone of DNA-PK necessary for the apoptotic response.
Effect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis.
Solier S, Kohn KW, Scroggins B, Xu W, Trepel J, Neckers L, Pommier Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Aug 7;109(32):12866-72
Proceedings of the National Academy of Sciences of the United States of America 2012 Aug 7;109(32):12866-72
Effect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis.
Hendrix A, Maynard D, Pauwels P, Braems G, Denys H, Van den Broecke R, Lambert J, Van Belle S, Cocquyt V, Gespach C, Bracke M, Seabra MC, Gahl WA, De Wever O, Westbroek W
Journal of the National Cancer Institute 2010 Jun 16;102(12):866-80
Journal of the National Cancer Institute 2010 Jun 16;102(12):866-80
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hsp90 Beta (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Hsp90 Beta Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA3-012-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hsp90 Beta (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Hsp90 Beta Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA3-012-A488) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
