Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- MA1-10372 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSP90 Monoclonal Antibody (MBH90AB)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody recognizes the epitope EEEVE within N-terminal part of ubiquitously expressed Hsp90 alpha and Hsp90 beta intracellular proteins with calculated Mw of 84.7 kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducing SDS-PAGE conditions.
- Reactivity
- Human, Mouse, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MBH90AB
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSP90 alpha/beta: purified HSP alpha (lane 1), purified HSP beta (lane 2), MCF-7 lysate (lane 3) and MCF-7 treated with UV (lane 4). Samples were probed with anti-HSP90 alpha/beta monoclonal antibody (Product # MA1-10372).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HSP90 was achieved by transfecting U-87 MG cells with HSP90 specific siRNAs (Silencer® select Product # s6994, s6995). Western blot analysis (Fig. a) was performed using whole cell extracts from the HSP90 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with HSP90 Polyclonal Antibody (Product # MA1-10372, 1 µg/mL dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HSP90.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), HEK 293T (Lane 3), HeLa (Lane 4), K562 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-HSP90 Polyclonal Antibody (Product # MA1-10372, 1 µg/mL dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 80 kDa band corresponding to HSP90 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of Hsp90 alpha and beta protein in MCF-7 cells by antibody MBH90AB Monoclonal antibody (Product # MA1-10372) to both Hsp90 alpha and beta isoform.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human testis (paraffin sections) using anti-HSP90 alpha/beta (MBH90AB) Monoclonal antibody (Product # MA1-10372).