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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-27420 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSP90 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2, RK3E, 4T1, SW480. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (94%), Pig (100%), Chicken (94%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSP90 beta Polyclonal Antibody (Product # PA5-27420). Sample (30 µg of whole cell lysate). A: 293T. B: A431. 7.5% SDS PAGE. HSP90 beta Polyclonal Antibody (Product # PA5-27420) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HSP90 beta was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HSP90 beta Polyclonal Antibody (Product # PA5-27420) at a dilution of 1:1500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HSP90 beta was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HSP90 beta Polyclonal Antibody (Product # PA5-27420) at a dilution of 1:1500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSP90 beta Polyclonal Antibody (Product # PA5-27420). Sample (30 µg of whole cell lysate). A: 293T. B: A431. 7.5% SDS PAGE. HSP90 beta Polyclonal Antibody (Product # PA5-27420) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HSP90/Heat Shock Protein 90 beta in methanol-fixed HCT116 cells using a HSP90/Heat Shock Protein 90 beta polyclonal antibody (Product # PA5-27420) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HSP90 beta antibody detects HSP90 beta protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HSP90 beta antibody (Product # PA5-27420) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HSP90 beta antibody detects HSP90 beta protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. HSP90 beta antibody (Product # PA5-27420) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using HSP90 beta (Product # PA5-27420) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
