GMA-012

antibody from Green Mountain Antibodies

Targeting: F8 DXS1253E, F8C, FVIII, HEMA

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Antibody Data

Product number

GMA-012

Provider

Green Mountain Antibodies

Product name

Anti-Factor VIII

Antibody type

Monoclonal

Antigen

Factor VIII

Description

GMA-012 is a versatile A2 domain specific antibody useful for detection of Factor VIII by western blot, ELISA, and immunoprecipitation. It is also used for affinity purification of Factor VIII from plasma. GMA-012 is frequently cited in literature under the name of R8B12. It pairs well with all of the 8000 series GMA antibodies (with the best pairing with GMA-8016 for sandwich ELISA). Wakabayashi, H et al. describes a sandwich ELISA of GMA-012 with GMA-8002 in reference 7.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

GMA-012

Vial size

0.1mg, 0.5mg

References

Submitted references

Activated factor X cleaves factor VIII at arginine 562, limiting its cofactor efficiency.

Plantier JL, Rolli V, Ducasse C, Dargaud Y, Enjolras N, Boukerche H, Négrier C
Journal of thrombosis and haemostasis : JTH 2010 Feb;8(2):286-93

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Factor VIII lacking the C2 domain retains cofactor activity in vitro.

Wakabayashi H, Griffiths AE, Fay PJ
The Journal of biological chemistry 2010 Aug 13;285(33):25176-84

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Enhanced factor VIII heavy chain for gene therapy of hemophilia A.

Chen L, Lu H, Wang J, Sarkar R, Yang X, Wang H, High KA, Xiao W
Molecular therapy : the journal of the American Society of Gene Therapy 2009 Mar;17(3):417-24

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Epitope mapping factor VIII A2 domain by affinity-directed mass spectrometry: residues 497-510 and 584-593 comprise a discontinuous epitope for the monoclonal antibody R8B12.

Ansong C, Miles SM, Fay PJ
Journal of thrombosis and haemostasis : JTH 2006 Apr;4(4):842-7

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Phenotypic correction of a mouse model of hemophilia A using AAV2 vectors encoding the heavy and light chains of FVIII.

Scallan CD, Liu T, Parker AE, Patarroyo-White SL, Chen H, Jiang H, Vargas J, Nagy D, Powell SK, Wright JF, Sarkar R, Kazazian HH, McClelland A, Couto LB
Blood 2003 Dec 1;102(12):3919-26

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Revision of the International Health Regulations. Progress Report, July 1997.

Releve epidemiologique hebdomadaire / Section d'hygiene du Secretariat de la Societe des Nations = Weekly epidemiological record / Health Section of the Secretariat of the League of Nations 1997 Jul 18;72(29):213-5