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LearnPA5-20025
antibody from Invitrogen Antibodies
Targeting: ANP32A
C15orf1, I1PP2A, LANP, MAPM, mapmodulin, PHAPI, PP32
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-20025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHAP I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~32kDa. A suggested positive control is Raji cell lysate. PA5-20025 can be used with blocking peptide PEP-0144.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Raji cell (A), mouse (B) and rat (C) testis tissue llysates using a PHAP I polyclonal antibody (Product # PA5-20025) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in (A) Human Raji Cells , (B) Mouse testis tissue lysate and (C) Rat testis tissue lysate. Loading: 15 µg of lysates per lane. Antibodies: PHAP I Polyclonal Antibody (Product # PA5-20025) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PHAP I Polyclonal Antibody (Product # PA5-20025) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PHAP I Polyclonal Antibody (Product # PA5-20025) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse small intestine cells using a PHAP I polyclonal antibody (Product # PA5-20025) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Small Intestine Cells labeling PHAP I with PHAP I Polyclonal Antibody (Product # PA5-20025) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse Small Intestine Tissue using PHAP I Polyclonal Antibody (Product # PA5-20025) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
