NBP1-33713
antibody from Novus Biologicals
Targeting: SET
2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- NBP1-33713 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33713, RRID:AB_2301540
- Product name
- Rabbit Polyclonal SET Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Antibody reactive against recombinant protein, detects immunogen. Further validation in progress.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer.
Ludvigsen M, Thorlacius-Ussing L, Vorum H, Moyer MP, Stender MT, Thorlacius-Ussing O, Honoré B
International journal of molecular sciences 2020 May 14;21(10)
International journal of molecular sciences 2020 May 14;21(10)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SET Antibody [NBP1-33713] - Western blot analysis of SET on Molt-4 lysates using NBP1-33713 at a dilution of 1:1000. 30 ug whole cell lysate loaded on 12% SDS PAGE gel.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw 264.7 whole cell lysate/extract. G. 30 µg C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg Molt-4 whole cell lysate/extract. F. 30 µg Raji whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.Observed SET protein is larger than the predicted M.W., possibly due to post-translational
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SET Antibody [NBP1-33713] - Immunohistochemical analysis of SET on paraffin-embedded DLD-1 xenograft, using NBP1-33713.